Example sentences for: premix

How can you use “premix” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Enzymes for the second-strand premix (Invitrogen) were added and incubated at 16°C for 2 h, followed by the addition of 2 units T4 DNA polymerase (Promega) and incubation at 16°C for 15 min before heat inactivation at 70°C for 10 min.

  • Subsequently, 25 μl of a premix containing 2 μl of 100 mM DTT (dithiothreitol), 10 μl 5X Superscript II buffer, 1 μl 50X aadNTP mix (25 mM dA, C, and GTP, 8.4 mM dTTP, and 16.

  • The mix was incubated at 65°C for 10 min before 5 μl first-strand premix was added followed by incubation at 42°C for 2 h.

  • The second-round second-strand synthesis, clean-up and IVT were carried out as for the first round, except that no ligase was added to the second-strand premix.

  • Cold second-strand premix (64.


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