Words similar to premix
Example sentences for: premix
How can you use “premix” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cold second-strand premix (64.
Six microliters first-strand premix were added and incubated at 42°C for 2 h (6 μl mix was made up with 2 μl 5× first-strand buffer, 1 μl 0.1 M DTT, 1 μl dNTPs (10 mM each), 0.3 μl T4gp32 (USB, 13.
Enzymes for the second-strand premix (Invitrogen) were added and incubated at 16°C for 2 h, followed by the addition of 2 units T4 DNA polymerase (Promega) and incubation at 16°C for 15 min before heat inactivation at 70°C for 10 min.
The second-round second-strand synthesis, clean-up and IVT were carried out as for the first round, except that no ligase was added to the second-strand premix.
The mix was incubated at 65°C for 10 min before 5 μl first-strand premix was added followed by incubation at 42°C for 2 h.