Words similar to preabsorbed

Example sentences for: preabsorbed

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No immunofluorescence was observed in tissue sections exposed to preabsorbed antisera or where the primary antiserum had been omitted (data not shown).
Controls included normal rabbit or mouse IgG, 1% BSA in PBS, or, in the case of BMPR antibodies, preabsorbed with the respective peptide used for immunization.
Briefly, specimens were washed with phosphate buffered saline (PBS) and incubated 20 minutes with mouse-anti human Thy-1, clone F10-44-1 [ 33 34 ] (5 μg/ml in PBS) reacting with placental villous pericytes, extravillous fibroblasts, and decidual cells [ 15 ] . After extensive washing in PBS, specimens were incubated 20 minutes with swine anti-mouse IgG peroxidase conjugate (SwAM; SEVAC, Prague, Czech Republic) diluted 1:50 and preabsorbed with rat kidney homogenate [ 15 ] . Antigen-antibody complexes were detected by standard diaminobenzidine technique (brown color).
Negative controls were carried out without primary antibodies and with primary antibodies preabsorbed (100-fold molar excess, 2 h, 25 C) with an 8-oxoguanine oligonucleotide (3850-100-01; Trevigen), human recombinant p53 (Santa Cruz Biotechnology, Santa Cruz, CA), Bcl-2 peptide (amino acids 41-54; StressGen), or recombinant polymerase β (Trevigen).
This was followed by peroxidaseconjugated and rat kidney preabsorbed swine anti-mouse IgG (SEVAC, Prague) or goat anti-mouse IgG and IgM (Jackson Immunoresearch), respectively.