Words similar to preabsorbed

Example sentences for: preabsorbed

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Specimens were then transferred to room temperature, extensively washed in freshly prepared PBS, pH 7.22, and incubated 20 minutes with swine anti-mouse IgG peroxidase conjugate (SwAM; SEVAC Praha, Prague, Czech Republic) diluted 1:20, and preabsorbed with rat kidney homogenate to remove nonspecific background [ 46 ] . Control slides were similarly processed, but primary antibody was replaced with PBS.
No immunofluorescence was observed in tissue sections exposed to preabsorbed antisera or where the primary antiserum had been omitted (data not shown).
Negative controls were carried out without primary antibodies and with primary antibodies preabsorbed (100-fold molar excess, 2 h, 25 C) with an 8-oxoguanine oligonucleotide (3850-100-01; Trevigen), human recombinant p53 (Santa Cruz Biotechnology, Santa Cruz, CA), Bcl-2 peptide (amino acids 41-54; StressGen), or recombinant polymerase β (Trevigen).
This was followed by peroxidaseconjugated and rat kidney preabsorbed swine anti-mouse IgG (SEVAC, Prague) or goat anti-mouse IgG and IgM (Jackson Immunoresearch), respectively.
In western blots, the 3B5 antibody identifies six distinct bands migrating between ~92 and 48 kDa, and this reactivity with protein extracts from all three species diminishes when the antibody is preabsorbed with porcine ovaries.