Words similar to preabsorbed

Example sentences for: preabsorbed

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This was followed by peroxidaseconjugated and rat kidney preabsorbed swine anti-mouse IgG (SEVAC, Prague) or goat anti-mouse IgG and IgM (Jackson Immunoresearch), respectively.
No immunofluorescence was observed in tissue sections exposed to preabsorbed antisera or where the primary antiserum had been omitted (data not shown).
Briefly, specimens were washed with phosphate buffered saline (PBS) and incubated 20 minutes with mouse-anti human Thy-1, clone F10-44-1 [ 33 34 ] (5 μg/ml in PBS) reacting with placental villous pericytes, extravillous fibroblasts, and decidual cells [ 15 ] . After extensive washing in PBS, specimens were incubated 20 minutes with swine anti-mouse IgG peroxidase conjugate (SwAM; SEVAC, Prague, Czech Republic) diluted 1:50 and preabsorbed with rat kidney homogenate [ 15 ] . Antigen-antibody complexes were detected by standard diaminobenzidine technique (brown color).
In western blots, the 3B5 antibody identifies six distinct bands migrating between ~92 and 48 kDa, and this reactivity with protein extracts from all three species diminishes when the antibody is preabsorbed with porcine ovaries.
Controls included normal rabbit or mouse IgG, 1% BSA in PBS, or, in the case of BMPR antibodies, preabsorbed with the respective peptide used for immunization.