Words similar to pre-steady

Example sentences for: pre-steady

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In L1, the residue at position 224 is a serine [ 35 ] , and the substrate-binding model for L1 predicts that this serine residue interacts with the carboxylate on substrate via a water molecule [ 37 ] . To test the proposed role of Ser224 in L1, serine was changed to an alanine (S224A), aspartic acid (S224D), and lysine (S224K), and these mutants were characterized using metal analyses, CD spectroscopy, steady-state kinetics, and pre-steady state kinetic studies.
However, in L1, Asn233 is 14 Å away from the modeled position of the substrate β-lactam carbonyl [ 37 ] . To test the role of Asn233 in substrate binding, the Asn was changed to a leucine (N233L) and to an aspartic acid (N233D), and these mutants were characterized by using metal analyses, CD spectroscopy, steady-state kinetics, and pre-steady state kinetic studies.