Words similar to pre-coated

Example sentences for: pre-coated

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For some experiments, the transduced cells were then activated with 5 μM ionomycin and 50 ng/ml PMA for 4 h or stimulated for 18 h by immobilized anti-CD3 (pre-coated at 10 μg/ml in PBS) or 5 μg/ml OVA peptide with antigen-presenting cells before analysis.
Using these membranes, filtration binding assays were performed according to previously described protocols [ 28 ] . To reduce nonspecific binding, 96-well filtration plates (fiberglass FB filters; Millipore, Bedford, USA) were pre-coated as suggested by the manufacturer with 0.5% polyethyleneimine + 0.1% BSA and then washed four times with 0.1% BSA.
Pdx1, Cdx1, and FoxA3 expression was induced in “stage 3,” when cell clusters were allowed to adhere to pre-coated wells at a density of 40–80 NSs/cm 2 and cultured in low-glucose medium with 2 μM RA for 2 wk (see Figure 1A and 1C).
Plates were pre-coated with Fn (10 μg/ml) for at least 2 hours at RT in 0.1 M NaHCO 3 . BSA (2% w/v) was then added to block unbound sites.
2 ml for association/dissociation studies and 0.75 ml in saturation and competition experiments) were then transferred to the pre-coated 96-well filtration plates (Millipore fiberglass FB filters), filtered and washed over a vacuum manifold with six 300 μl per well washes of binding buffer supplemented with 2% hydoxypropyl cyclodextrin (HPCD; Sigma; ST Louis, USA) + 0.1% BSA to decrease non-specific binding.