Words similar to pre-absorbed

Example sentences for: pre-absorbed

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After overnight incubation, membranes were washed and incubated with peroxidase labeled secondary antibody (goat anti-mouse IgG and IgM, pre-absorbed with human serum and diluted 1: 2000; Jackson Immunoresearch, West Grove, PA) for 1 hour at room temperature.
After extensive washing, the slides were incubated with biotinated donkey anti-goat polyclonal antibody (DAKO) pre-absorbed with rat kidney homogenate [ 15 ] , followed by peroxidase-coupled streptavidin, and the peroxidase visualized by diaminobenzidine as recommended by the kit (LSAB) vendor (DAKO).
After several washes in PBS (room temperature), the sections were incubated with peroxidase labeled secondary antibody - goat anti-mouse IgG and IgM, pre-absorbed with human serum (Jackson Immunoresearch, West Grove, PA), diluted 1:50 and absorbed with rat kidney homogenate as described [ 39 ] . Tissue sections were processed further as described previously [ 40 ] , including hematoxylin counterstaining of the nuclei.
When the ERβ antibody was pre-absorbed with blocking peptide (PA/ERβ), no bands were detected (Fig.