Words similar to postcoated

Example sentences for: postcoated

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To measure the binding of biotinyl-sCD4 and biotinyl-gp120 (in the presence and absence of sCD4), respectively, to CAP, the biotinylated proteins were added to CAP and control postcoated wells without CAP, respectively, at dilutions shown in Fig.
To detect binding of CD4 to CAP treated and control gp120, wells coated with graded amounts of gp120 and postcoated as described above were reacted with biotinylated sCD4 (1 μg/well) in PBS containing 100 μg/ml BSA for 18 h at 4°C, washed with TS and the bound biotinylated CD4 was determined after adding HRP-streptavidin (1 μg/ml) in TS containing 0.25% gelatine and 0.05% Tween 20 for 30 min at 37°C.
In other experiments, wells were coated with CAP (1 μg/ml) in 25 mM sodium acetate, pH 6.0 and postcoated as described above.
Subsequently the wells were washed and postcoated with BSA and gelatin as described above, except that these proteins were dissolved in 0.14 M NaCl, 0.01 M Tris, pH 7.0 (TS).
Subsequently, the wells were washed and postcoated for 1 h at 20°C with bovine serum albumin (BSA) and gelatin (1 and 0.1 mg/ml in 0.14 M NaCI, 0.01 M Tris, pH 7.0 [TS]).