Example sentences for: postcoated

How can you use “postcoated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To measure the binding of biotinyl-sCD4 and biotinyl-gp120 (in the presence and absence of sCD4), respectively, to CAP, the biotinylated proteins were added to CAP and control postcoated wells without CAP, respectively, at dilutions shown in Fig.

  • To detect binding of CD4 to CAP treated and control gp120, wells coated with graded amounts of gp120 and postcoated as described above were reacted with biotinylated sCD4 (1 μg/well) in PBS containing 100 μg/ml BSA for 18 h at 4°C, washed with TS and the bound biotinylated CD4 was determined after adding HRP-streptavidin (1 μg/ml) in TS containing 0.25% gelatine and 0.05% Tween 20 for 30 min at 37°C.

  • In other experiments, wells were coated with CAP (1 μg/ml) in 25 mM sodium acetate, pH 6.0 and postcoated as described above.

  • Subsequently, the wells were washed and postcoated for 1 h at 20°C with bovine serum albumin (BSA) and gelatin (1 and 0.1 mg/ml in 0.14 M NaCI, 0.01 M Tris, pH 7.0 [TS]).

  • Subsequently the wells were washed and postcoated with BSA and gelatin as described above, except that these proteins were dissolved in 0.14 M NaCl, 0.01 M Tris, pH 7.0 (TS).


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