Words similar to post-hybridization
Example sentences for: post-hybridization
How can you use “post-hybridization” in a sentence? Here are some example sentences to help you improve your vocabulary:
Quality control criteria were as described on our web site ( http://microarray.cnmcresearch.org, link to "programs in genomic applications"), and included sufficient cRNA amplification, and adequate post-hybridization scaling factors.
These differences include: increased precision in spiked transcript concentration through dilution from the same IVT product; utilization of Test2 chips reflecting current probe sets and production chemistry; no post-hybridization streptavidin amplification; multiple measurements per condition; comparison of transcript intensities obtained with absent or present complex cRNA background; and the use of commercially available Test2 arrays rather than custom-made arrays containing hundreds of probe pairs per gene.
This has the advantages of allowing simultaneous assay of two or more differently labeled probes and detection directly after post-hybridization washes.
It is possible to argue that post-hybridization amplification would improve detection, but obviously at the expense of potentially saturating expression levels of more abundant genes.
For those BACs that demonstrated significant cross-hybridizaton to other chromosomal regions, FISH was repeated using higher stringency (60% formamide) hybridization solution and post-hybridization washes.