Example sentences for: post-hybridization

How can you use “post-hybridization” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Composition and pH of hybridization mix and post-hybridization wash buffer pH were important in these instances.

  • This has the advantages of allowing simultaneous assay of two or more differently labeled probes and detection directly after post-hybridization washes.

  • For those BACs that demonstrated significant cross-hybridizaton to other chromosomal regions, FISH was repeated using higher stringency (60% formamide) hybridization solution and post-hybridization washes.

  • a chromogenic stain following application of intermediary reagents) rather than biochemical alteration of DNA-DNA interactions (measured by spectrophotometry) [ 8 ] . It was also concluded that mismatched probe targets should be removed using empirically derived post-hybridization washing conditions in order to achieve specificity and that appropriate conditions should be determined by experiment rather than calculated theoretically from T m . In a later study Herrington et al.

  • Hybridization conditions and post-hybridization washes determine ISH assay stringency, i.e. the number of matched/mismatched sequences permitted between a probe and its target.


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