Words similar to post-hybridization
Example sentences for: post-hybridization
How can you use “post-hybridization” in a sentence? Here are some example sentences to help you improve your vocabulary:
a chromogenic stain following application of intermediary reagents) rather than biochemical alteration of DNA-DNA interactions (measured by spectrophotometry) [ 8 ] . It was also concluded that mismatched probe targets should be removed using empirically derived post-hybridization washing conditions in order to achieve specificity and that appropriate conditions should be determined by experiment rather than calculated theoretically from T m . In a later study Herrington et al.
Hybridization conditions and post-hybridization washes determine ISH assay stringency, i.e. the number of matched/mismatched sequences permitted between a probe and its target.
Composition and pH of hybridization mix and post-hybridization wash buffer pH were important in these instances.
These differences include: increased precision in spiked transcript concentration through dilution from the same IVT product; utilization of Test2 chips reflecting current probe sets and production chemistry; no post-hybridization streptavidin amplification; multiple measurements per condition; comparison of transcript intensities obtained with absent or present complex cRNA background; and the use of commercially available Test2 arrays rather than custom-made arrays containing hundreds of probe pairs per gene.
Quality control criteria were as described on our web site ( http://microarray.cnmcresearch.org, link to "programs in genomic applications"), and included sufficient cRNA amplification, and adequate post-hybridization scaling factors.
Loading...