Words similar to post-fixed
Example sentences for: post-fixed
How can you use “post-fixed” in a sentence? Here are some example sentences to help you improve your vocabulary:
The L4 and L5 spinal nerve roots and lumbar spinal cords (a 2 mm segment centered at the L5 root entry level) were dissected out and post-fixed with 2% osmium tetroxide in 100 mM cacodylate buffer, pH 7.6.
Whole-mount in situ hybridization with Ptc1 probe and X-gal staining for whole-mount β-galactosidase detection were performed as described [ 26 ] . For histology, embryos stained with X-gal were post-fixed in 4% paraformaldehyde, wax-embedded, and 20 μm sections were prepared.
1) and post-fixed in 1% aqueous osmium tetroxide for 24 hours at 4°C.
After cryo-sectioning, the sections were post-fixed in paraformaldehyde and then subjected to in situ hybridization (ISH) with digoxigenin-labeled probes (Dig RNA Labeling Kit, Roche), followed by alkaline phosphatase-mediated detection.
In some cases, after mounting onto untreated culture dishes, the sections were post-fixed with 10% formalin for 30 to 45 minutes at 4ºC and rinsed three times with Ham's F12 medium (Sigma).
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