Example sentences for: post-fixed

How can you use “post-fixed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Sixteen micron thick sections were mounted on gelatin-coated slides and post-fixed in 4% paraformaldehyde for 30 minutes at room temperature.

  • The L4 and L5 spinal nerve roots and lumbar spinal cords (a 2 mm segment centered at the L5 root entry level) were dissected out and post-fixed with 2% osmium tetroxide in 100 mM cacodylate buffer, pH 7.6.

  • Sixteen micron thick sections were mounted on gelatin-coated slides and were post-fixed in 4% paraformaldehyde for 30 minutes at room temperature.

  • After cryo-sectioning, the sections were post-fixed in paraformaldehyde and then subjected to in situ hybridization (ISH) with digoxigenin-labeled probes (Dig RNA Labeling Kit, Roche), followed by alkaline phosphatase-mediated detection.

  • Re-hydrated and post-fixed embryos were incubated for 1 h in hybridization buffer (50% formamide, 4× SSC, and 0.01% Tween 20).


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