Example sentences for: pombe

How can you use “pombe” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Transformation frequency is the standard assay for plasmid origin activity in S. pombe [ 9, 12, 13].

  • Since wild-type S. pombe has 44 crossovers per meiosis (between 11 and 18 per chromosome) [ 36 ] , the mutants statistically have less than 0.1 crossovers per chromosome per meiosis and are thus achiasmatic.

  • These two regions could not be well aligned and we could not predict a common structure with comparative analysis with ten Eucaryotic sequences in 1988 (see Figures 35-43 in [ 48 ] ). However, once a larger number of related Eucaryotic 23S-like rRNA sequences was determined, we reanalyzed these two regions and were able to align those regions to other related organisms ( e.g., S. cerevisiae with Schizosaccharomyces pombe, Cryptococcus neoformans, Pneumocystis carinii, Candida albicans, and Mucor racemosus ) and predict a secondary structure that is common for all of these rRNAs (see Figures 3and 6 in [ 52 ] ). While the secondary structures for the fungal 23S-like rRNAs are determined in these regions, the animal rRNAs were only partially solved.

  • pombe his5 +PCR amplification/ transformation method was used [ 62 ] . Correct integrants were verified by PCR using primers that amplified a DNA fragment that spanned the recombination junction.

  • The promoter derived from the S. pombe ADH1 gene exhibits specific spacing requirements for its function such that the permissive distance between the TATA element and the transcription initiation site is at most 130 bp [ 19 20 ] . A greater distance results in transcription initiation at an alternative site and inactivation of URA3 expression.


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