Words similar to polyvinylidene
Example sentences for: polyvinylidene
How can you use “polyvinylidene” in a sentence? Here are some example sentences to help you improve your vocabulary:
Gels were transferred in 20% methanol to polyvinylidene difluoride membrane (Amersham Pharmacia Biotech, Piscataway, NJ, USA).
PIP3: Phosphatidyl-3,4,5-triphosphate; DMEM: Dulbecco's Modified Eagle's Medium; PI-3Kinase: phosphatidylinositol-3-kinase; MAPK: mitogen-activated protein kinase; IGF: insulin-like growth factor; PTEN: phosphatase and tensin homolog deleted from chromosome 10; SOS: son-of-sevenless ras exchange factor; ASK: apoptosis stimulating kinase; FKHR: forkhead related protein; cFLIP: cellular FLICE interacting protein; BSA: bovine serum albumin; TBS: tris buffered saline; PVDF: polyvinylidene difluoride
Protein lysates were collected for Western blot analysis as described elsewhere [ 17 35 39 ] . Briefly, following separation by SDS-PAGE, the proteins (20 μg/lane) were transferred (100 V, 1 h) to polyvinylidene difluoride membranes.
AID (α subunit interaction domain); BID (β subunit interaction domain); DMSO (dimethyl sulfoxide); EGTA (ethylene glycol bis-aminoethylether tetraacetic acid); HEPES (2-hydroxylethyl piperazine 2-ethane sulfonic acid); MOPS (3N-Morpholino-propane sulfonic acid); PVDF (polyvinylidene difluoride); TTX (tetrodotoxin); TEA (tetraethylamonium).
For in vitro protein-protein interaction assays, 5 μg of either GST-CAF1 or GST purified proteins (prepared as described in [ 25 ] ) were subjected to 10% SDS/PAGE and transferred onto a polyvinylidene difluoride membrane (Millipore) by electroblotting.