Words similar to polb
Example sentences for: polb
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Vero (ATCC, passage 15-20), MRC-5 (ATCC, passage 12-15) and PolB3 (kind gift of D. Coen, Harvard University, Boston MA) cell lines were grown in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal calf serum (FCS) and incubated at 37°C, 5%CO 2 . PolB3 represents a Vero cell line constitutively expressing the HSV-1 Pol [ 17 ] . PCV was synthesized at SmithKline Beecham Pharmaceuticals.
HSV-1 strain SC16 (wild type laboratory virus), HSV-2 SB5 (wild type laboratory virus), HSV-2 6652 (wild type clinical virus), HSV-2 83D (wild type clinical virus), HSV-2 6757 (high error mutant clinical virus) were described previously [ 21 ] and the HSV-1 PAA r5 (antimutator laboratory virus) utilized in this study was a kind gift of D. Coen (Harvard University, Boston, MA) [ 10 ] . HP66 (HSV-1 genome containing a LacZ insertion within the pol coding region) viral DNA was kindly provided by D. Coen (Harvard University, Boston, MA) [ 17 ] . HP66 viral stocks were prepared by transfecting HP66 viral DNA into PolB3 cells, which allow for virus propagation by expressing the viral polymerase.