Words similar to plx

Example sentences for: plx

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Net1N(7m) contained mutations required to strongly inhibit Plx1-dependent disruption of Net1N-Cdc14 complexes, whereas Net1N(19m') contained additional mutations without compromising the affinity of Net1N to Cdc14 [ 25 ] . Both of these mutant proteins, when expressed in E. coli and assembled with recombinant Cdc14, were found to be severely defective in releasing Cdc14 upon treatment with Plx1 (data not shown).
Thus, the mutant RENT is at least 30-fold more resistant to Plx1 than wild-type RENT.
Mutant RENT complexes retrieved from these cells were significantly more resistant to the Polo-like kinase Plx1 in release assays (Fig.
Active but not inactive Plx1 phosphorylated both Net1N and Cdc14, and completely dislodged Net1N from bead-bound Cdc14 (Figure 4B).
We constructed an extensive set of phospho-site mutants of Net1N, which were tested for their ability to remain bound to Cdc14 beads in the presence of Plx1 [ 25 ] . By successively combining individual mutations in Cdc5 phosphorylation sites, we created the phosphosite mutants Net1N(7m) (with S30, S31, S48, S60, S64, S242, and S335 all changed to A) and Net1N(19m') (with T16, S30, S31, S48, S60, S64, T194, T195, T196, T197, S223, S224, S228, S231, S242, S295, S301, T302, S335 all changed to A).