Words similar to plx

Example sentences for: plx

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Mutant RENT complexes retrieved from these cells were significantly more resistant to the Polo-like kinase Plx1 in release assays (Fig.
For experiments in Figure 5, all four reactions consisted of 125 ng GST-T7-Cdc14 adsorbed to 20 μl anti-GST beads, 200 ng His6-T7-Net1N, 1 μl Plx1, 20 μl kinase buffer containing ATP, and the ATP depletion system (see below).
Active but not inactive Plx1 released a significant portion of Cdc14 and Sir2 from bead-bound Net1 to the supernatant fraction (Figure 4C).
Thus, we conclude that like Cdc5, the protein kinase activity of Plx1 can disrupt the interaction between Net1N and Cdc14.
RENT complexes from net1▲ NET1-myc9 and net1▲ net1(7m)-myc9 cell lysates were captured on 9E10 beads and treated with increasing amounts of recombinant Plx1 plus ATP.