Words similar to plx

Example sentences for: plx

How can you use “plx” in a sentence? Here are some example sentences to help you improve your vocabulary:

Myc9-Net1 and the associated RENT subunits were immunopurified from extracts of myc9-NET1 cells on 9E10 beads and treated with Plx1 plus ATP.
Mutant RENT complexes retrieved from these cells were significantly more resistant to the Polo-like kinase Plx1 in release assays (Fig.
GST-T7-Cdc14 (RDB 872) and GST-Cdc14 (RDB 1183) were expressed in E. coli BL21(DE3) pLysS cells, and purified on glutathione-agarose beads (Sigma) as described previously [ 33 ] . His6-T7-Net1(1-341) (Net1N, RDB1153) [ 11 ] was expressed in E. coli BL21(DE3) pLysS cells, and purified on Ni-NTA agarose beads (Qiagen) as described previously for p28 Kix1 [ 34 ] . Purification of His6-Plx1, His6-Plx1(N172), and His6-HA-Cdc5 from baculovirus-infected insect cells followed an earlier protocol [ 24 ] . The concentrations of purified proteins were in the ranges of: Net1N~0.
In total, we tested more than 20 phospho-site mutants of Net1, and identified two - Net1(7m) and Net1(19m') - that still bound Cdc14 normally, but displayed a strong defect in releasing Cdc14 upon treatment with Plx1.
Next, we asked if the mutant RENT complex is more resistant toward Plx1.