Words similar to plx
Example sentences for: plx
How can you use “plx” in a sentence? Here are some example sentences to help you improve your vocabulary:
Indeed, Cdc5 and the Xenopus polo-like kinase Plx1 reduced the binding affinity between recombinant Cdc14 and Net1 by phosphorylating Net1, and dislodged both Cdc14 and Sir2 from the RENT complex in vitro .
Thus, we conclude that like Cdc5, the protein kinase activity of Plx1 can disrupt the interaction between Net1N and Cdc14.
We constructed an extensive set of phospho-site mutants of Net1N, which were tested for their ability to remain bound to Cdc14 beads in the presence of Plx1 [ 25 ] . By successively combining individual mutations in Cdc5 phosphorylation sites, we created the phosphosite mutants Net1N(7m) (with S30, S31, S48, S60, S64, S242, and S335 all changed to A) and Net1N(19m') (with T16, S30, S31, S48, S60, S64, T194, T195, T196, T197, S223, S224, S228, S231, S242, S295, S301, T302, S335 all changed to A).
Myc9-Net1 and the associated RENT subunits were immunopurified from extracts of myc9-NET1 cells on 9E10 beads and treated with Plx1 plus ATP.
RENT complexes from net1▲ NET1-myc9 and net1▲ net1(7m)-myc9 cell lysates were captured on 9E10 beads and treated with increasing amounts of recombinant Plx1 plus ATP.