Words similar to plx

Example sentences for: plx

How can you use “plx” in a sentence? Here are some example sentences to help you improve your vocabulary:

Mutant RENT complexes retrieved from these cells were significantly more resistant to the Polo-like kinase Plx1 in release assays (Fig.
Myc9-Net1 and the associated RENT subunits were immunopurified from extracts of myc9-NET1 cells on 9E10 beads and treated with Plx1 plus ATP.
Net1N(7m) contained mutations required to strongly inhibit Plx1-dependent disruption of Net1N-Cdc14 complexes, whereas Net1N(19m') contained additional mutations without compromising the affinity of Net1N to Cdc14 [ 25 ] . Both of these mutant proteins, when expressed in E. coli and assembled with recombinant Cdc14, were found to be severely defective in releasing Cdc14 upon treatment with Plx1 (data not shown).
We constructed an extensive set of phospho-site mutants of Net1N, which were tested for their ability to remain bound to Cdc14 beads in the presence of Plx1 [ 25 ] . By successively combining individual mutations in Cdc5 phosphorylation sites, we created the phosphosite mutants Net1N(7m) (with S30, S31, S48, S60, S64, S242, and S335 all changed to A) and Net1N(19m') (with T16, S30, S31, S48, S60, S64, T194, T195, T196, T197, S223, S224, S228, S231, S242, S295, S301, T302, S335 all changed to A).
In total, we tested more than 20 phospho-site mutants of Net1, and identified two - Net1(7m) and Net1(19m') - that still bound Cdc14 normally, but displayed a strong defect in releasing Cdc14 upon treatment with Plx1.