Words similar to plx
Example sentences for: plx
How can you use “plx” in a sentence? Here are some example sentences to help you improve your vocabulary:
To address this question, we tested the binding affinity of combinations of Plx1-phosphorylated or unphosphorylated Net1N and Cdc14.
For experiments in Figure 5, all four reactions consisted of 125 ng GST-T7-Cdc14 adsorbed to 20 μl anti-GST beads, 200 ng His6-T7-Net1N, 1 μl Plx1, 20 μl kinase buffer containing ATP, and the ATP depletion system (see below).
Active but not inactive Plx1 phosphorylated both Net1N and Cdc14, and completely dislodged Net1N from bead-bound Cdc14 (Figure 4B).
RENT complexes from net1▲ NET1-myc9 and net1▲ net1(7m)-myc9 cell lysates were captured on 9E10 beads and treated with increasing amounts of recombinant Plx1 plus ATP.
Indeed, Cdc5 and the Xenopus polo-like kinase Plx1 reduced the binding affinity between recombinant Cdc14 and Net1 by phosphorylating Net1, and dislodged both Cdc14 and Sir2 from the RENT complex in vitro .
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