Example sentences for: plx

How can you use “plx” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • We constructed an extensive set of phospho-site mutants of Net1N, which were tested for their ability to remain bound to Cdc14 beads in the presence of Plx1 [ 25 ] . By successively combining individual mutations in Cdc5 phosphorylation sites, we created the phosphosite mutants Net1N(7m) (with S30, S31, S48, S60, S64, S242, and S335 all changed to A) and Net1N(19m') (with T16, S30, S31, S48, S60, S64, T194, T195, T196, T197, S223, S224, S228, S231, S242, S295, S301, T302, S335 all changed to A).

  • Because recombinant Plx1 but not Cdc5 was consistently expressed at high levels in insect cells, and because release assays using Plx1 and Cdc5 yielded similar results, we used Plx1 in most of our release assays.

  • Thus, we conclude that like Cdc5, the protein kinase activity of Plx1 can disrupt the interaction between Net1N and Cdc14.

  • Next, we asked if the mutant RENT complex is more resistant toward Plx1.

  • RENT complexes from net1▲ NET1-myc9 and net1▲ net1(7m)-myc9 cell lysates were captured on 9E10 beads and treated with increasing amounts of recombinant Plx1 plus ATP.


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