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Example sentences for: planaria
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planaria differ from other models used to study morphogenesis (insects, nematodes, fish, amphibians and mammals) by their phenomenal ability to regenerate [ 1 ] . Moreover, planaria are used for in vivo tumorogenicity studies of pure compounds and complex environmental mixtures, and for mechanistic studies of tumorogenesis [ 2 ] .
Several DNA sequences similar to the mariner element have been reported in G. tigrina [ 10 16 ] . There are about 7000 copies of this element in the genome, which appear to code for an active transposase [ 10 ] . None of these mariner elements are present in the genomes of other turbellarians, including the species closest to G. tigrina [ 10 ] . With the aid of PCR using specific primers for the planarian mariner element, we isolated and sequenced several clones from sexual and asexual planaria.
Although asexual strains of planaria exist independently for a long time, they display substantial genetic resemblance.
The second strain, collected in Russia near Moscow, represented sexual planaria capable of both sexual and asexual reproduction.
Total genomic DNA (100 mg) from planaria was dissolved in 100 μl TN buffer (10 mM Tris-HCl, pH 8.0; 5 mM NaCl), and mixed with two volumes of 0.2 M NaOH with 1% SDS, followed by 1.5 volumes of 5 M potassium acetate (pH 4.8).
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