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Example sentences for: piscataway

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Nitrocellulose membranes (Hybond ECL), secondary antibodies, enhanced chemiluminescence (ECL) reagent, and film (Hyperfilm ECL) were obtained from Amersham Pharmacia Biotech (Piscataway, NJ).
Forty-eight hours after transfection, cells were lysed and membrane and supernatant fractions harvested as described previously [ 8 ] . 10 μg of membrane proteins and normalized volumes of the supernatants were resolved by SDS-polyacrylamide electrophoresis (10%), transferred to nitrocellulose, and probed with a monoclonal antibody to the EE epitope [ 34 ] . The antigen-antibody complexes were visualized with ECL chemiluminescence (Amersham Biosciences, Piscataway, NJ).
Genomic DNA samples were obtained from (a) National Institute of Mental Health (NIMH) Center for Genetic Studies, Rutgers University Cell and DNA Repository, Piscataway, NJ and (b) Coriell Cell Repository (HD 100 CAU).
Bound antibodies were detected by a chemiluminescent detection system (ECL; Amersham Pharmacia Biotech, Piscataway, NJ) as recommended by the manufacturer's protocol . For exposure (first 5 min, and second up to 2 h) we used Kodak XAR film (Eastman Kodak, Rochester, NY).
The following additional polymers were obtained from commercial sources likely to be different from those for proprietary products being developed as microbicides: carrageenans κ and λ (Sigma, St. Louis, MO; mixed at a 1:1 (w/w) ratio in all experiments); cellulose sulfate (Across Organics, Piscataway, NJ); dextran sulfate (M.W.