Words similar to pili
Example sentences for: pili
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In view of these lines of evidence suggesting that nontypeable H influenzae grows as a biofilm during human respiratory tract infection, the goals of the present study are to 1) Develop an assay to study biofilms of nontypeable H. influenzae in vitro; 2) Characterize the extent to which isolates from the middle ear fluid of children with otitis media and from the sputum of adults with COPD form biofilms; 3) Begin to elucidate the characteristics of the outer membrane antigens of nontypeable H. influenzae during growth as a biofilm; and 4) Preliminarily assess the role of pili in biofilm formation.
For example, an increase in asialo-GM 1 glycoproteins on the apical surface of CF airway epithelial cells provides greater opportunity for adhesion by the type IV pili of P. aeruginosa [ 3 7 8 ] . Furthermore, the first extracellular domain of wild type CFTR has been found to act as a P. aeruginosa -specific receptor, which is involved in the clearance of P. aeruginosa from the airways via epithelial cell desquamation [ 9 10 11 ] , a process greatly impaired in the CF lung.
Nontypeable H. influenzae strain M37 and an isogenic variant designated M37-1 which is deficient in expression of pili were kindly provided by Robert Munson [ 34 ] .
This observation parallels that made in several other bacterial species showing the pili are critical in the early stages of biofilm formation [ 36 41 ] .
Strain M37-1 lacks expression of pili as indicated by absence of hemagglutination and absence of a 22 kDa band on SDS-PAGE corresponding to pilin [ 34 ] . In an experiment comparing biofilm formation in 4 duplicate wells, strain M37-1, the pilus deficient variant, yielded an average optical density of 0.204 whereas its isogenic parent strain, M37, yielded an average optical density of 0.884.