Words similar to photolithographic
Example sentences for: photolithographic
How can you use “photolithographic” in a sentence? Here are some example sentences to help you improve your vocabulary:
Alternatively, microarrays can be composed of short oligonucleotides (25 bases) synthesized directly onto a solid matrix using photolithographic technology (Affymetrix) [ 2, 9] or constructed from long oligonucleotides (55-70 bases) spotted onto glass slides [ 10, 11, 12].
cDNA microarrays are created by spotting amplified cDNA fragments in a high density pattern onto a solid substrate such as a glass slide [ 1 2 ] . Oligonucleotide arrays are either spotted or constructed by chemically synthesizing approximately 25-mer oligonucleotide probes directly onto a glass or silicon surface using photolithographic technology [ 3 ] .
At the time of this study, Affymetrix synthesizes 25-mer oligonucleotides on a 640 × 640 array with 20 μm feature size using photolithographic fabrication techniques (some data used in this study were collected from some previous generation arrays of 540 × 540 cells and 24 μm feature size).
Affymetrix oligonucleotide arrays are created by a combination of DNA synthesis and photolithographic techniques, whereas cDNA arrays are constructed by spotting or printing PCR products or oligonucleotides onto glass slides [ 6, 7, 8, 9].
The cDNA array has a long history of development [ 3 ] stemming from immunodiagnostic work in the 1980s; however, it has been most widely developed in recent years by Stanford University (California) researchers depositing cDNA tags onto glass slides, or chips, with precise robotic printers [ 4 ] . Labeled cDNA fragments are then hybridized to the tags on the chip, scanned, and differences in mRNA between samples identified and visualized using a variation of the red/green matrix originally introduced by Eisen and colleagues [ 5 ] . The light-generated oligonucleotide array, developed by Affymetrix, Inc. (Santa Clara, CA), involves synthesizing short 25-mer oligonucleotide probes directly onto a glass slide using photolithographic masks [ 6 7 ] . Sample processing includes the production of labeled cRNA, hybridization to a microarray, and quantification of the obtained signal after laser scanning.
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