Example sentences for: phosphohistone

How can you use “phosphohistone” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The precipitated phosphohistone was collected by centrifugation at 3000 × g for 5 min.

  • However, as seen in Figure 1, the dephosphorylation of phosphohistone by the catalytic subunit of purified PP5 from bovine brain is also potently inhibited by okadaic acid.

  • TCA was added to precipitate the phosphohistone, and this precipitation-resuspension wash was repeated 5 times.

  • 32P-labeled phosphohistone was made as described [ 14 15 18 ] . When using phosphohistone as substrate, reactions were carried out in 80 μl followed by quantification of the liberated 32Pi by phosphomolybdate extraction assay [ 14 15 18 ] . Reactions were followed with time, and results were corrected by subtraction of the corresponding values from an enzyme-free reaction.

  • Using phosphohistone as substrate, the native Pf enzyme exhibited a specific activity of 210 ± 20 nmoles of 32P per minute per mg enzyme, which is comparable to that of the recombinant enzyme.


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