Words similar to phosphohistone

Example sentences for: phosphohistone

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32P-labeled phosphohistone was made as described [ 14 15 18 ] . When using phosphohistone as substrate, reactions were carried out in 80 μl followed by quantification of the liberated 32Pi by phosphomolybdate extraction assay [ 14 15 18 ] . Reactions were followed with time, and results were corrected by subtraction of the corresponding values from an enzyme-free reaction.
Phosphatase activity against pNPP or phosphohistone substrates was determined essentially as described previously.
However, as seen in Figure 1, the dephosphorylation of phosphohistone by the catalytic subunit of purified PP5 from bovine brain is also potently inhibited by okadaic acid.
Phosphatase activity against phosphohistone was determined by the quantification of [ 32P] liberation from phosphohistone as described previously [ 34].
TCA was added to precipitate the phosphohistone, and this precipitation-resuspension wash was repeated 5 times.