Words similar to phosphohistone
Example sentences for: phosphohistone
How can you use “phosphohistone” in a sentence? Here are some example sentences to help you improve your vocabulary:
TCA was added to precipitate the phosphohistone, and this precipitation-resuspension wash was repeated 5 times.
This procedure yields phosphohistone with a specific activity > 4.5 × 10 6CPM/nMole incorporated phosphate.
The precipitated phosphohistone was collected by centrifugation at 3000 × g for 5 min.
32P-labeled phosphohistone was made as described [ 14 15 18 ] . When using phosphohistone as substrate, reactions were carried out in 80 μl followed by quantification of the liberated 32Pi by phosphomolybdate extraction assay [ 14 15 18 ] . Reactions were followed with time, and results were corrected by subtraction of the corresponding values from an enzyme-free reaction.
Phosphatase activity against pNPP or phosphohistone substrates was determined essentially as described previously.