Words similar to phosphocellulose

Example sentences for: phosphocellulose

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Phosphocellulose-purified tubulin (PC-tubulin) free of microtubule-associated proteins (MAPs) was obtained by warm/cold polymerization/depolymerization with the addition of a final phosphocellulose chromatography step to separate tubulin from MAPs [ 37 38 ] . Tubulin concentrations were determined spectrophotometrically, using ε 278 = 1.2 L/(g cm) [ 39 ] .
Induction of the enzyme protein was carried out as described before for the wild type HIV-1RT [ 29 ] . The enzyme with the hexahistidine-tag was purified from bacterial lysates by immobilized metal affinity chromatography [ 33 ] , while non-hexahistidine-tagged enzyme was purified using the phosphocellulose and Q-Sepharose columns as described previously [ 24 ] .