Example sentences for: phospho-site

How can you use “phospho-site” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • RENT complexes comprised of Net1 phospho-site mutants are resistant to disassembly by Polo-like kinase, but exhibit only minor phenotypes in vivo

  • First, net1(7m) and net1(19m') mutants exerted a modest effect on release of Cdc14 from the nucleolus, in that ~40% of cells with long mitotic spindles retained focal localization of Cdc14 and that both net1 phospho-site mutants displayed a modest defect in 'Cdc14 early anaphase release' (FEAR; [ 26 ] ). Second, net1(19m') showed a synthetic growth defect when combined with dbf2-1 or the msd2-1 allele of CDC5 . Third, phosphorylation site mapping of Net1 purified from arrested cdc14-1 cells (WS and SC, unpublished data) revealed that at least one (S231) of the five in vivo phosphorylation sites in the first 341 amino acids was directly phosphorylated by Cdc5 in vitro . Nevertheless, because expression of neither Net1(7m) nor Net1(19m') had a major impact on exit from mitosis, any direct effect of Cdc5 on Net1 appears to be minor, and the requirement for Cdc5 in the release of Cdc14 might arise principally from phosphorylation of other release-promoting proteins.

  • We constructed an extensive set of phospho-site mutants of Net1N, which were tested for their ability to remain bound to Cdc14 beads in the presence of Plx1 [ 25 ] . By successively combining individual mutations in Cdc5 phosphorylation sites, we created the phosphosite mutants Net1N(7m) (with S30, S31, S48, S60, S64, S242, and S335 all changed to A) and Net1N(19m') (with T16, S30, S31, S48, S60, S64, T194, T195, T196, T197, S223, S224, S228, S231, S242, S295, S301, T302, S335 all changed to A).

  • In total, we tested more than 20 phospho-site mutants of Net1, and identified two - Net1(7m) and Net1(19m') - that still bound Cdc14 normally, but displayed a strong defect in releasing Cdc14 upon treatment with Plx1.

  • There are two extreme interpretations of our analysis of the Net1(7m) and Net1 (19m') mutants: First, Cdc5 is not directly involved in disrupting the RENT complex, and second, Cdc5 directly disassembles the RENT complex via phosphorylation of Net1, but because it is not feasible to evaluate all possible 10 9(=2 30) combinations of phospho-site mutants or because the sites phosphorylated by Cdc5 in vivo are different from those in vitro due to the presence of factors such as Sir2, Pol I, and rDNA [ 10 23 ] , we missed key phosphorylation sites that would have given a more definitive phenotype when mutated.

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