Example sentences for: phip

How can you use “phip” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To determine if the Rv0365c gene was responsible for the increased survival of sur2 bacteria, the full-length M. tuberculosis Rv0365c ORF as well as a truncated ORF similar to that present in the insert in the sur2 clone were subcloned into the pHIP vector downstream of the M. tuberculosis hsp65 promoter.

  • Plasmid pHIP13 was created by cloning a Bgl II-digested PCR fragment containing Rv2233 , Rv2234 , and Rv2235 into the Bam HI site downstream of the hsp65 promoter of pHIP.

  • Because the hsp65 promoter in pHIP contains a ribosome binding site (rbs), the spacing between the rbs and the start codon of the cloned gene is about the same in these constructs as the spacing between the rbs and the start codon of the hsp65 gene in wild-type M. tuberculosis.

  • Subcloning ORFs into pHIP and pBPhin

  • This amplicon was Not I-digested and cloned into the Not I site of the pHIP1 plasmid containing the xylE gene to generate pHIP4.


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