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Example sentences for: philips
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For electron microscopy imaging, ultra-thin sections were stained with uranyl acetate and lead citrate (5 minutes each) and viewed under a Philips CM10 transmission electron microscope.
1% gelatin, 1% normal goat serum, 0.3% Triton-X-100 in TBS), placed in primary antibody (1:10 diluted in blocking buffer for all antibodies used) for 2 hours at room temperature, rinsed 3 times for 5 min each in TBS, placed in gold-conjugated secondary antibodies (10 nm gold anti-mouse and 5 nm gold anti-rabbit) for 1 hour, rinsed 3 times in TBS, fixed in 2% glutaraldehyde, rinsed 3 times in TBS, rinsed in water, stained with Reynold=s lead citrate followed by aqueous 2% uranyl acetate, dried on filter paper and viewed using a Philips CM10 transmission electron microscope.
They were then blot-dried and examined with a Philips CM 10 transmission electron microscope for localization of the colloidal gold probe.
The scan was performed from the apex to the diaphragm using a Tomoscan SR 7000 (Philips, Eindhoven) and a semi-quantitative assessment of parenchymal consolidation in ZEEP was performed according to a technique previously described [ 4, 5, 8, 9].
Some researchers have reported the ability of CD56 positive T cells to exhibit spontaneous killing against some of the targets such as K562 or P815, upon stimulation with IL-2 [ 22 23 24 25 ] . However Lanier and Philips who carried out extensive studies on the LAK phenomenon have emphasised that although CD16+ T cells exhibit non-MHC-restricted cytotoxicity to some targets upon IL-2 stimulation, their contribution was quantitatively and qualitatively very minimal as compared to the NK subset [ 7 11 ] . The differences in the ability of lymphocytes of different phenotypes to respond to IL-2 stimulation and carry out spontaneous lysis of different targets could probably be attributed to the differential expression of inhibitory receptors present on the targets such as KIR and CD94:NKG2 [ 26 ] .
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