Words similar to pharmingen

Example sentences for: pharmingen

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Apoptotic status of cells was assayed using Annexin V-FITC Apoptosis Detection Kit for annexin V/ propidium iodide cell labeling and using Apo-Direct Kit for terminal deoxynucleotidyltransferase dUTP-FITC nick end labeling (TUNEL) according to manufacturer's instruction (BD Pharmingen, San Diego, CA).
Five μg of total RNA was hybridized to the in vitro transcribed hAPO-4 probe set (PharMingen, SanDiego, CA), and the RPA assay was performed according to the manufacturer's protocol.
The samples were transferred on to nitrocellulose membranes, which were incubated in blocking buffer (TBS, pH 7.5, containing 10% dried milk and 0.1% Tween-20) and then rabbit anti-Bid antiserum (BD PharMingen, San Diego, CA), 1:2000 dilution in the same blocking buffer, at 4°C, overnight.
Total IgE was measured in duplicate with mouse serum at 1:100 dilution by sandwich ELISA (PharMingen) according to the manufacturer's instructions [ 18 ] .
Ten micrograms of total RNA from each sample was used for RNase protection assay (mCK -2b and -3b Multi-Probe Template Sets, PharMingen, San Diego, CA, USA), exposed to a Phosphor screen, and quantified by a Phosphorimager with the use of Image Quant software (Molecular Dynamics, Sunnyvale, CA, USA).