Words similar to pharmingen
Example sentences for: pharmingen
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Aliquots of 50 μL were incubated for 20 min at room temperature with 2 μL of annexin V-FITC from BD Pharmingen (San Diego, CA) and saturating concentrations of PE-conjugated Mab.
Four-color flow cytometry was performed using the following monoclonal antibodies (Pharmingen): biotin anti-mouse CD25 (clone: 7D4) visualized with Per-CP conjugated streptavidin; APC anti-mouse CD44 (clone: IM7); and three phycoerythrin (PE) conjugated antibodies: anti-mouse CD4 (clone: RM4-5), anti-mouse CD8a (clone: 53-6.
Antibodies: SKP2 (Zymed 32-3300, 1:250 for immunoblotting), SKP2 (Santa Cruz sc-7164, 1:500 for immunofluorescence staining), p27 (Transduction Laboratories K25030, 1:5000 for immunoblotting, 1:1000 for immunofluorescence staining), cyclin A (Neomarkers Ab-6, MS-1061, 1:100 for immunoblotting), cyclin E (Neomarkers Ab-1, RB-012, 2 ug per immunoprecipitation) cyclin E (Neomarkers Ab-2, MS-870, 1:200 for immunoblotting), HRT1 (affinity-purified rabbit polyclonal, gift from R. Deshaies, 1:1000 for immunoblotting), CUL1 (Zymed 71-8700, 1:250 for immunoblotting), SKP1 (Transduction laboratories 610530, 1:5000 for immunoblotting), JAB1/CSN5 (GeneTex MS-Jab11-PX1, 1:1000 for immunoblotting), RB1 (Pharmingen 554136, 1:500 for immunoblotting), CDK2 (Santo Cruz Biotechnology sc-163, 2 ug per immunoprecipitation, 1:500 for immunoblotting), Tubulin (Sigma T5168, 1:2000 for immunoblotting).
Anti-β1 blocking antibody (Ha2/5) was from PharMingen.
Apoptotic status of cells was assayed using Annexin V-FITC Apoptosis Detection Kit for annexin V/ propidium iodide cell labeling and using Apo-Direct Kit for terminal deoxynucleotidyltransferase dUTP-FITC nick end labeling (TUNEL) according to manufacturer's instruction (BD Pharmingen, San Diego, CA).
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