Example sentences for: pharmingen

How can you use “pharmingen” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After washing with Perm/Wash buffer three times (first wash being a 1 hr period in which the cells remain in Perm/Wash buffer for 1 hr on ice), cells were incubated with phycoerythrin-labeled streptavidin (Pharmingen) for 1 hr on ice, and washed three times as described for the Ab incubation step, then brought to 1 ml with PBS [ 28 ] .

  • Serum Ig values were interpolated from standard curves obtained by coating the plates directly with purified IgG1 or IgG2a (PharMingen) at a starting concentration of 500 ng/ml, according to the manufacturer's instructions.

  • Sections were processed for antibody staining using the following antibodies: rabbit [ 86 ] and sheep (BioDesign) anti-SOD1 (both recognize mouse and human SOD1), mouse anti-cytochrome c oxidase subunit CO1 and CO4 (Molecular Probes, OR), rabbit anti-TOM20 and TOM40 (Santa Cruz Biotechnology, CA), mouse anti-cytochrome c (BD PharMingen, San Diego, CA), sheep anti-catalase (The Binding Site, Birmingham, England), rabbit anti-catalase (Calbiochem, SanDiego, CA), rabbit anti-PMP70 (ABR, Golden, CO or Axxora, SanDiego, CA), and rabbit anti-cathepsin D (DAKO, Carpinteria, CA) according to the protocol described previously [ 26 28 ] . The stained sections were examined and digitized using a confocal microscope (Leica TCS-SP).

  • Anti-β1 blocking antibody (Ha2/5) was from PharMingen.

  • Immunofluorescence staining of cell surface markers was performed using FITC-labeled antibodies against CD3, B220, CD11b, and CD11c (BD PharMingen).


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