Words similar to pharmingen
Example sentences for: pharmingen
How can you use “pharmingen” in a sentence? Here are some example sentences to help you improve your vocabulary:
The cells were stained with directly conjugated mAbs to human cytokines (Pharmingen) and analyzed by flow cytometry [ 17].
Serum Ig values were interpolated from standard curves obtained by coating the plates directly with purified IgG1 or IgG2a (PharMingen) at a starting concentration of 500 ng/ml, according to the manufacturer's instructions.
After washing with Perm/Wash buffer three times (first wash being a 1 hr period in which the cells remain in Perm/Wash buffer for 1 hr on ice), cells were incubated with phycoerythrin-labeled streptavidin (Pharmingen) for 1 hr on ice, and washed three times as described for the Ab incubation step, then brought to 1 ml with PBS [ 28 ] .
Antibodies: SKP2 (Zymed 32-3300, 1:250 for immunoblotting), SKP2 (Santa Cruz sc-7164, 1:500 for immunofluorescence staining), p27 (Transduction Laboratories K25030, 1:5000 for immunoblotting, 1:1000 for immunofluorescence staining), cyclin A (Neomarkers Ab-6, MS-1061, 1:100 for immunoblotting), cyclin E (Neomarkers Ab-1, RB-012, 2 ug per immunoprecipitation) cyclin E (Neomarkers Ab-2, MS-870, 1:200 for immunoblotting), HRT1 (affinity-purified rabbit polyclonal, gift from R. Deshaies, 1:1000 for immunoblotting), CUL1 (Zymed 71-8700, 1:250 for immunoblotting), SKP1 (Transduction laboratories 610530, 1:5000 for immunoblotting), JAB1/CSN5 (GeneTex MS-Jab11-PX1, 1:1000 for immunoblotting), RB1 (Pharmingen 554136, 1:500 for immunoblotting), CDK2 (Santo Cruz Biotechnology sc-163, 2 ug per immunoprecipitation, 1:500 for immunoblotting), Tubulin (Sigma T5168, 1:2000 for immunoblotting).
For analysis of intracellular CTLA-4, spleen cells were first stained with the above fluorescence-labeled antibodies, fixed and rendered permeable, and stained with R-phycoerythrin (PE)-labeled anti-CTLA-4 using a Cytofix/Cytoperm kit (BD PharMingen).
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