Example sentences for: pharmacia

How can you use “pharmacia” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Membranes were washed with TBST and then incubated for 1 hour at room temperature with a secondary antibody followed by washing three times with TBST, immersed in ECL Plus western blotting detection system (Amersham Pharmacia Biotech, Buckinghamshire, England) for 1 min and then exposed to Kodak X-ray film (Rochester, NY).

  • Induction of GST-PNUTS fusion protein synthesis and isolation by affinity chromatography using glutathione-Sepharose beads (Pharmacia) was performed as described previously [ 32 ] . Induced LLWO2F cell extracts were then incubated for 1 hour at 4°C with recombinant GST or GST-PNUTS fusion protein bound to glutathione-Sepharose.

  • The radiolabeled nucleotide [α- 32P]dCTP (3,000 Ci/mmol) and nick translation kits were purchased from Amersham-Pharmacia.

  • To examine whether mactinin was present in immune complexes in rheumatoid arthritis fluid, some samples were acidified by dialysis against 0.1 M sodium acetate, pH 4.1, to dissociate complexes [ 18 ] . The acidified samples were first fractionated on an 800 ml G-75 Sephadex (Pharmacia, Piscataway, NJ) size-exclusion column.

  • Blots were developed with Enhanced Chemiluminescence (Amersham Pharmacia Biotech).


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