Words similar to pfpp

Example sentences for: pfpp

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RNA interference (RNAi), mediated by short interfering double-stranded RNA molecules (siRNA or dsRNA), is now recognized as a major mechanism of post-transcriptional gene silencing (PTGS) in essentially all eukaryotes [ 16 ] . Recently, the technique has been successfully applied to cultured mammalian cells, whereby introduction of 21-nucleotide long synthetic dsRNA molecules corresponding to specific mRNA sequences effectively and specifically degraded the cognate mRNAs and abrogated the expression of the corresponding proteins [ 17 18 ] . This prompted us to test a similar approach to knockdown PfPP1 function in the erythrocytic P. falciparum stages.
The success in ablating PfPP1 is particularly gratifying to us on a number of accounts.
3, a protein band of approximately 37,000 Mr was produced from the pET-15b-PfPP1 clone, which is in agreement with the predicted molecular weight of 34,904 of PfPP1 and roughly another 2,000 added for the (His) 6 region of pET-15b (Novagen: Madison, WI).
This motif is also present in PfPP5 (Fig.
SDS-PAGE and Western blot using chemiluminescence-based detection were performed essentially as described [ 15 ] . Immunoprecipitation of PfPP5 complexes followed by immunoblot analysis of the precipitate were carried out essentially as described [ 34 ] .