Words similar to pfpp

Example sentences for: pfpp

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Expression of native PfPP1
Thus, in comparing the full sequences of the PP5s, only three of the four PfPP5 TPRs were aligned with the others, and the second TPR of PfPP5 was left out.
Expression and catalytic properties of PfPP1
First, PfPP5 contained the catalytic core found in all Ser/Thr phosphatases of the PPP family, including the signature motifs such as GDXHGQ, GDFVDRG, RGNHE, HGLL, and SAPNYCD, to name a few [ 22 23 ] . Site-directed mutagenesis and structural studies in PP 1 and PPλ have previously established the roles of specific amino acid residues in these domains in the various aspects of catalysis, such as metal ion binding, phosphate recognition, and co-ordination of water molecules [ 24 25 26 ] .
To express the putative PfPP5 protein, we subcloned it in the bacterial expression vector pET-15b and introduced the plasmids into E. coli BL21(DE3).