Words similar to pestis
Example sentences for: pestis
How can you use “pestis” in a sentence? Here are some example sentences to help you improve your vocabulary:
PcrG and His 6 -LcrV were coexpressed in a Y. pestis strain with lcrG and lcrV deleted and the cleared lysates were applied to a Talon column.
This result demonstrated that PcrG and LcrV interact in Y. pestis . The interaction of PcrG with LcrV supports the mutagenesis and sequence data indicating that residues conserved between LcrG and PcrG are likely to be important for interaction with LcrV.
Yersinia pestis , the causative agent of bubonic plague, harbors a large virulence plasmid that is essential for pathogenicity.
Mutazyme (Stratagene, La Jolla, CA) was used to amplify PcrG for cloning into pBAD18 [ 33 ] . The ligation reaction was electroporated into lcrG Y. pestis (which is temperature sensitive due to its Ca 2+-blind phenotype) and the cells were plated on TMH medium [ 29 ] containing arabinose, Ca 2+, kanamycin and carbenicillin.
Additionally, we investigated whether the Pseudomonas homolog of LcrG, PcrG, could transcomplement an lcrG strain of Y. pestis . The opportunistic pathogen Pseudomonas aeruginosa utilizes a type III secretion system for intracellular delivery of toxins [ 21 22 23 ] that is the most similar to the Yersinia system [ 21 ] . Several components of the Pseudomonas system have been demonstrated to functionally complement genetic defects in the Yersinia homologs including the LcrV homolog, PcrV [ 24 25 ] . Homologs of LcrG and LcrV (i.e.