Words similar to pestis
Example sentences for: pestis
How can you use “pestis” in a sentence? Here are some example sentences to help you improve your vocabulary:
Since PcrG and LcrV interacted in Y. pestis , the ability of PcrG to complement an lcrG strain of Y. pestis was examined.
Only two of the 560 protein-coding genes that show clear orthology between E. coli and Buchnera would have been removed (due to absence from both Vibrio cholerae and Yersinia pestis ). The two genes ( dnaC and dnaT ) are linked in both Buchnera and E. coli and were retained in the ancestor.
Additionally, we investigated whether the Pseudomonas homolog of LcrG, PcrG, could transcomplement an lcrG strain of Y. pestis . The opportunistic pathogen Pseudomonas aeruginosa utilizes a type III secretion system for intracellular delivery of toxins [ 21 22 23 ] that is the most similar to the Yersinia system [ 21 ] . Several components of the Pseudomonas system have been demonstrated to functionally complement genetic defects in the Yersinia homologs including the LcrV homolog, PcrV [ 24 25 ] . Homologs of LcrG and LcrV (i.e.
To further understand the phenotypes obtained, the ability of LcrG S23R and L30R to interact with LcrV was examined in Y. pestis . Wild type LcrG, LcrG S23R, LcrG L30R, and LcrG A16R were independently co-expressed with His 6 -tagged LcrV in a strain of Y. pestis deleted for lcrGV . The cells were French pressed and the cleared lysates were applied to Talon columns to purify His 6 -tagged LcrV.
The interaction of LcrG with LcrV is a critical factor in controlling the activity of the type III secretion system in Y. pestis [ 20 ] . In order to gain a deeper understanding of this critical interaction, we determined the region of LcrG required for interaction with LcrV.
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