Example sentences for: pestis

How can you use “pestis” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • PcrG and His 6 -LcrV were coexpressed in a Y. pestis strain with lcrG and lcrV deleted and the cleared lysates were applied to a Talon column.

  • This result demonstrated that PcrG and LcrV interact in Y. pestis . The interaction of PcrG with LcrV supports the mutagenesis and sequence data indicating that residues conserved between LcrG and PcrG are likely to be important for interaction with LcrV.

  • Yersinia pestis , the causative agent of bubonic plague, harbors a large virulence plasmid that is essential for pathogenicity.

  • Mutazyme (Stratagene, La Jolla, CA) was used to amplify PcrG for cloning into pBAD18 [ 33 ] . The ligation reaction was electroporated into lcrG Y. pestis (which is temperature sensitive due to its Ca 2+-blind phenotype) and the cells were plated on TMH medium [ 29 ] containing arabinose, Ca 2+, kanamycin and carbenicillin.

  • Additionally, we investigated whether the Pseudomonas homolog of LcrG, PcrG, could transcomplement an lcrG strain of Y. pestis . The opportunistic pathogen Pseudomonas aeruginosa utilizes a type III secretion system for intracellular delivery of toxins [ 21 22 23 ] that is the most similar to the Yersinia system [ 21 ] . Several components of the Pseudomonas system have been demonstrated to functionally complement genetic defects in the Yersinia homologs including the LcrV homolog, PcrV [ 24 25 ] . Homologs of LcrG and LcrV (i.e.


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