Example sentences for: perm

How can you use “perm” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cells were fixed in Fix & Perm Medium A (Caltag) for 10 min at room temperature.

  • After washing twice in PBS, cells were resuspended in Fix & Perm Medium B (Caltag) and allowed to remain at room temperature for 30 min.

  • Cells were washed three times in Perm/Wash buffer, then incubated with 1 μg biotinylated anti-rat IL-6 (Pharmingen)/10 6cells in 100 l Perm/Wash buffer for 1 hr on ice.

  • After washing with Perm/Wash buffer (Pharmingen), cells were incubated in 5-10 mg/ml goat Ig for 1 hr on ice.

  • After washing with Perm/Wash buffer three times (first wash being a 1 hr period in which the cells remain in Perm/Wash buffer for 1 hr on ice), cells were incubated with phycoerythrin-labeled streptavidin (Pharmingen) for 1 hr on ice, and washed three times as described for the Ab incubation step, then brought to 1 ml with PBS [ 28 ] .


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