Words similar to perfect-match

Example sentences for: perfect-match

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The absolute signal distributions of the six oligonucleotide arrays can be seen in Figure 2for the HIV (perfect-match (PM) distributions) and glnA (Cy3 G and Cy5 R distributions) experiments both before and after normalization.
There are two common experimental platforms for expression profiling; redundant oligonucleotide arrays (Affymetrix GeneChips) [ 1 ] , and spotted cDNA microarrays [ 2 3 4 ] . The Affymetrix GeneChips have the inherent advantages of redundancy, specificity, and transportability; there are typically 30-40 oligonucleotide probes (features) designed against each gene tested by the array, with paired perfect-match and mismatch probes, with standardized factory synthesis of arrays [ 5 6 ] . The uniform nature of the arrays permits databasing of individual profiles, which facilitates comparison of data generated by different laboratories.
Each pair consists of a 'perfect-match' primer and a 'mismatch' primer that has a single base change from the perfect match and is meant to control for nonspecific hybridization effects.
The resulting hybridization intensity measurements for each oligonucleotide were averaged across all hybridizations and scaled as a fraction of the average intensity of the perfect-match oligonucleotide for each set.
The photolithographically synthesized oligonucleotide microarrays that underlie the Affymetrix GeneChip ®array use pairs (typically 16 or 20) of perfect-match (PM) and mismatch (MM) features.