Words similar to perfect-match
Example sentences for: perfect-match
How can you use “perfect-match” in a sentence? Here are some example sentences to help you improve your vocabulary:
Each pair consists of a 'perfect-match' primer and a 'mismatch' primer that has a single base change from the perfect match and is meant to control for nonspecific hybridization effects.
The photolithographically synthesized oligonucleotide microarrays that underlie the Affymetrix GeneChip ®array use pairs (typically 16 or 20) of perfect-match (PM) and mismatch (MM) features.
The resulting hybridization intensity measurements for each oligonucleotide were averaged across all hybridizations and scaled as a fraction of the average intensity of the perfect-match oligonucleotide for each set.
For each gene (probe set), the Affymetrix software calculates an 'average difference' between the intensities of the perfect-match and mismatch primers, and additionally makes an overall determination as to the absence or presence of a gene (absent/present call) in the sample.
There are two common experimental platforms for expression profiling; redundant oligonucleotide arrays (Affymetrix GeneChips) [ 1 ] , and spotted cDNA microarrays [ 2 3 4 ] . The Affymetrix GeneChips have the inherent advantages of redundancy, specificity, and transportability; there are typically 30-40 oligonucleotide probes (features) designed against each gene tested by the array, with paired perfect-match and mismatch probes, with standardized factory synthesis of arrays [ 5 6 ] . The uniform nature of the arrays permits databasing of individual profiles, which facilitates comparison of data generated by different laboratories.
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