Words similar to percp
Example sentences for: percp
How can you use “percp” in a sentence? Here are some example sentences to help you improve your vocabulary:
For staining, two or three fluorochromes (PE, FITC, PerCP) (PharMingen) were used to gate on the CD3, CD4 and CD8 T lymphocyte population or CD11c +veDC in single cell suspensions from tumor nodule.
Cell populations were stained with anti-CD3-APC, CD14-FITC, CD19-PerCP and CD56-PE (Becton Dickinson), and quantitated by flow cytometry (FacsCalibur, Becton Dickinson).
Polyclonal goat anti-murine MIG/CXCL9 and anti-murine IP-10/CXCL10 specific anti-serum were produced and characterized as previously described [ 31 ] . Anti-mouse IFNγ monoclonal (R4-462, American Type Culture Collection, Rockville, Maryland, MD) neutralizing antibody was purified by affinity chromatography from SCID mice ascites, which was generated 3-4 weeks after intraperitoneal injection of 10 6R4-462 hybridoma cells per mouse [ 32 ] . For flow cytometry, labeled antibodies (FITC, PE, PerCP) against the T cell surface markers to CD3, CD4, CD8 and against the DC markers, CD11c and DEC 205 and the appropriate controls were purchased from PharMingen.
Then 10 ul of fluorescein isothiocyanate (FITC) mouse anti-CD8 (Leu-2a) (Becton-Dickinson), and 10 ul peridinin chlorophyll (PerCP) mouse anti-CD3 (Leu-4) (Becton-Dickinson) were added, followed by incubation in the dark for 20 min at room temperature.
In addition, cells were stained with a combination of anti-CD3 PerCP, anti-CD8 FITC and anti CD4 PE (Becton Dickinson, San Jose, CA) . To assess their proliferative ability and to confirm the specificity of tetramer staining of ex vivo CD3+ CD8+ T-cells, lymphocytes were cultured at a concentration of 3 × 10 6/ml in RPMI enriched with 10% human serum with addition of 10 μg/ml of the appropriate peptide and 20 U/ml of IL-2 for 7 days.
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