Words similar to percp

Example sentences for: percp

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For proliferation experiments, cells were stained with anti-BrdU FITC/anti-cytokine PE/CD4 PerCP (all antibodies from BD Immunocytometry Systems).
Polyclonal goat anti-murine MIG/CXCL9 and anti-murine IP-10/CXCL10 specific anti-serum were produced and characterized as previously described [ 31 ] . Anti-mouse IFNγ monoclonal (R4-462, American Type Culture Collection, Rockville, Maryland, MD) neutralizing antibody was purified by affinity chromatography from SCID mice ascites, which was generated 3-4 weeks after intraperitoneal injection of 10 6R4-462 hybridoma cells per mouse [ 32 ] . For flow cytometry, labeled antibodies (FITC, PE, PerCP) against the T cell surface markers to CD3, CD4, CD8 and against the DC markers, CD11c and DEC 205 and the appropriate controls were purchased from PharMingen.
Then 10 ul of fluorescein isothiocyanate (FITC) mouse anti-CD8 (Leu-2a) (Becton-Dickinson), and 10 ul peridinin chlorophyll (PerCP) mouse anti-CD3 (Leu-4) (Becton-Dickinson) were added, followed by incubation in the dark for 20 min at room temperature.
For this study, PBMC were treated as described above except that anti-human CD3 +(FL4) coupled to allophycocyanin (APC) was used along with anti-IFN-γ FITC (FL1), anti-CD28 PE (FL2) and anti-CDS PerCP (FL3).
Cell populations were stained with anti-CD3-APC, CD14-FITC, CD19-PerCP and CD56-PE (Becton Dickinson), and quantitated by flow cytometry (FacsCalibur, Becton Dickinson).