Words similar to peptone

Example sentences for: peptone

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The same crosses were also used to determine if Tab +was recessive or dominant: for every tab mutant, haploid cdc15Δ tab [pMET3-cdcl5-2, URA3] and diploid cdc15Δ/cdc15Δ tab/+ [pMET3-cdc15-2, URA3 ] cells were pre-grown on YPD (1% yeast extract/2% peptone + 2% glucose) at 25°C for 2-3 days, spotted in 5-fold serial dilutions onto SD+5-FOA plates, and the number of 5-FOA resistant colonies from the diploid was divided by that from the haploid to obtain ratio P. The mutant was defined to be dominant if P= 0.1-1, semi-dominant if P= 0.01-0.
All yeast strains used in this study are listed in Table 1. General yeast manipulations were conducted by standard methods [ 62 ] with transformations by the lithium acetate method [ 63 ] . All strains were grown at 23° in YPD (yeast extract, peptone, 2% glucose) unless otherwise noted.
Cells were grown in 1% yeast extract/ 2% peptone (YP) with 2% glucose (YPD), 2% galactose (YPG), or 2% raffinose (YPR).
The resulting colonies were then grown in YPAD media (1% yeast extract, 2% peptone, 0.04% adenine, and 2% glucose) and plated on 5-FOA plates to select clones emerging by a second recombination event that eliminated the URA3 gene together with the additional copy of PPT1 . To confirm gene replacement, chromosomal DNA from these clones was used for PCR amplification of the region encoding HA-Ppt1p.