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Example sentences for: pdj-

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To mark the regions of low-copy repeats in human 15q11-q13, we utilized a probe derived from the donor locus of a major component of the PWS/AS breakpoint duplication clusters, PAC clone pDJ-778A2 which contains a significant portion of the HERC2 gene in 15q13 [ 33 ] . In addition, we selected probes corresponding to sites distal to the PWS/AS domain flanking a large inverted-duplicated 15 (inv dup(15)) supernumerary marker chromosome [ 23 ] . Specifically, probe RPCI-11-88O16 (abbreviated RP11-88O16) was selected for FISH analysis because it lies distal to the inversion-duplication event breakpoint and is linked to STS marker D15S1010 [ 23 24 ] . Upon reaching the PTR inversion breakpoint interval with probe RP11-40J8, flanking BACs were chosen from the November 2002 human genome assembly to establish the extent of the interval and define the boundaries of the interval.
Previous cytogenetic analyses of human, chimpanzee, gorilla and orangutan G-banded metaphase chromosomes demonstrated that the pericentric inversion was a chimpanzee-specific event, with humans maintaining the ancestral state of this region when compared to gorilla and orangutan [ 1 2 ] . Two-color FISH experiments utilizing distal probe RP11-88O16, and the PWS/AS common deletion breakpoint-marking probe pDJ-778A2 clearly show that the chimpanzee lineage is the sole great ape species to harbor this rearrangement (Figure 2).