Words similar to pbst

Example sentences for: pbst

How can you use “pbst” in a sentence? Here are some example sentences to help you improve your vocabulary:

Biotinylated Ad5.CMV-LacZ, prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, was diluted in PBST and incubated for immobilization in streptavidin-coated wells (well diameter, 0.64 cm; Reacti-Bind Streptavidin Coated Polystyrene Wells, Pierce Chemical) (50 μl per well) for 2 h at 25°C with shaking on a rotary shaker at 150 rpm.
The wells were washed with PBST and then with PBS (pH 7.4) without Tween 20.
5 × 10 6viral particles in 50 μl PBST per well) and incubated with shaking on a rotary shaker at 150 nm at 25°C for 30 min.
Membranes were blocked in 5% BLOT-QuickBlocker PBST buffer (plus 0.1% Tween-20), washed three times with PBST buffer (plus 0.1% Tween-20), incubated in primary antibody diluted in femto/TBST buffer in a tray for 90 min at room temperature (or overnight at 4°C), washed five times (as above), incubated in secondary antibody-HRP (horseradish peroxidase)-conjugate (goat anti-rabbit) as above, washed four times with femto/TBST buffer followed by transfer to detection buffer and detection reagent.
These spot areas within plastic rings were treated with 1 mg/ml sulfo-NHS-LC-biotin in PBS (pH 7.4) (50 μl per spot) for 2 h at 4°C and then washed with PBST to remove unreacted biotinylation reagent.