Words similar to pbst

Example sentences for: pbst

How can you use “pbst” in a sentence? Here are some example sentences to help you improve your vocabulary:

The viral particles were subjected to three rounds of ultrafiltration with ZM-500 centrifugal filtration units (molecular mass cut-off, 500 kDa; Millipore) using PBS (pH 7.4) containing 0.05% Tween 20 (PBST) as a diluent to remove non-virion-associated biotinylation reagent.
Neutralite avidin (Southern Biotechnology Associates) (25 μg in 50 μl PBST per spot) was added to the spot areas within plastic rings, which had been treated with sulfo-NHS-LC-biotin, and allowed to bind to biotin moieties on the well surface.
The wells were washed with PBST and then with PBS (pH 7.4) without Tween 20.
Biotinylated Ad5.CMV-LacZ (1 × 10 7viral particles per spot), prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, were incubated in the spot areas within plastic rings at 25°C for 2 h, followed by washing the wells with PBST to remove unbound viral particles.
These spot areas within plastic rings were treated with 1 mg/ml sulfo-NHS-LC-biotin in PBS (pH 7.4) (50 μl per spot) for 2 h at 4°C and then washed with PBST to remove unreacted biotinylation reagent.