Words similar to pbst
Example sentences for: pbst
How can you use “pbst” in a sentence? Here are some example sentences to help you improve your vocabulary:
The viral particles were subjected to three rounds of ultrafiltration with ZM-500 centrifugal filtration units (molecular mass cut-off, 500 kDa; Millipore) using PBS (pH 7.4) containing 0.05% Tween 20 (PBST) as a diluent to remove non-virion-associated biotinylation reagent.
Neutralite avidin (Southern Biotechnology Associates) (25 μg in 50 μl PBST per spot) was added to the spot areas within plastic rings, which had been treated with sulfo-NHS-LC-biotin, and allowed to bind to biotin moieties on the well surface.
The wells were washed with PBST and then with PBS (pH 7.4) without Tween 20.
5 × 10 6viral particles in 50 μl PBST per well) and incubated with shaking on a rotary shaker at 150 nm at 25°C for 30 min.
Biotinylated Ad5.CMV-LacZ, prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, was diluted in PBST and added to streptavidin-coated wells (2.
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