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Example sentences for: palindromic
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For example a copy of GGTTT and its complement can form a sequence GG(N 5-6 )CC, fulfilling the minimal DNA binding requirement for the ZAS proteins other than a lack of the 5' guanine [ 25 ] . Similarly, the GT-rich RSS nonamer and the palindromic RSS heptamer can serve as binding sites of KRC.
To identify long-range palindromic structures, an RNA secondary structure algorithm in the program DNASIS version 2.0 was used.
Published methods include enterobacterial repetitive intergenic consensus sequence-PCR (ERIC-PCR), and repetitive intergenic palindromic sequence-PCR (REP-PCR) [ 2 3 ] ; random amplified polymorphic DNA-PCR (RAPD-PCR) or arbitrary primed-PCR (AP-PCR) [ 4 5 ] ; and restriction fragment length polymorphism-PCR (RFLP-PCR) of the omp 2 locus [ 6 7 ] . ERIC-PCR and RAPD-PCR are both affected by assay conditions and environmental effects during the amplification process [ 8 9 10 ] . Although the results are highly reproducible within a laboratory, laboratory-to-laboratory reproducibility has been problematic and thus makes the universal application of these methods unlikely (compare [ 2 ] and [ 3 ] ). RFLP-PCR of the omp 2 locus is not constrained by issues of reproducibility and has been useful for the differentiation of Brucella species [ 6 ] and for differentiation among isolates from marine mammal hosts [ 7 ] . However, as a tool for the epidemiology of brucellosis in livestock, this technique is limited by the low rate of natural sequence divergence within the locus at the biovar level.
This notion is supported by the presence, within the oligo probe, of nucleotides sequences 5'AGATCC 3' (n-98 to -93) and 5'ACTGGT 3' (n-85 to -80) which are identical or complementary to half of the palindromic ARE motif of prostatien C3 subunit gene (5'AGTACG 3') [ 35 ] and that of PSA promoter (5' AGTGCT 3') [ 20 ] , respectively.
The smad7 gene contains a perfect palindromic Smad binding element (GTCTAGAC) while the pai-1 and the pthrp promoters harbor AGAC tandem repeats [ 19 33 ] which binds Smad proteins less efficiently [ 34 ] . The upa gene contains only an AP1-binding site which resembles the Smad3/4-responsive AGAC motif [ 35 ] . A weaker binding site could require Smad3 to be present at higher concentrations for efficient binding and would make TGFβ-dependent transcription from a gene more vulnerable to reduced nuclear accumulation of the Smad3 protein.
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