Words similar to oxidase
Example sentences for: oxidase
How can you use “oxidase” in a sentence? Here are some example sentences to help you improve your vocabulary:
Sections were processed for antibody staining using the following antibodies: rabbit [ 86 ] and sheep (BioDesign) anti-SOD1 (both recognize mouse and human SOD1), mouse anti-cytochrome c oxidase subunit CO1 and CO4 (Molecular Probes, OR), rabbit anti-TOM20 and TOM40 (Santa Cruz Biotechnology, CA), mouse anti-cytochrome c (BD PharMingen, San Diego, CA), sheep anti-catalase (The Binding Site, Birmingham, England), rabbit anti-catalase (Calbiochem, SanDiego, CA), rabbit anti-PMP70 (ABR, Golden, CO or Axxora, SanDiego, CA), and rabbit anti-cathepsin D (DAKO, Carpinteria, CA) according to the protocol described previously [ 26 28 ] . The stained sections were examined and digitized using a confocal microscope (Leica TCS-SP).
Some of these proteins may be part of a multiprotein chromatin-modifying catalytic complex of which the SWIRM amino oxidase proteins are a part.
Although the location of activated NADPH oxidase complex in phagolysosomes has been reported, the release of either H 2 O 2 or O 2 •- in situ upon stimulation with PMA has not been directly shown in live cells [ 10 11 ] . In order to directly measure the oxidative activity in lysosomes, I use a reduced fluorescein derivative that is conjugated with BSA, OxyBURST Green H 2 HFF BSA, as shown in Figure 1. Spectrofluorimetric analysis indicates that this reduced compound is non-fluorescent, and becomes fluorescent upon HRP-catalyzed oxidation.
Respiratory chain enzymes were measured in 10% homogenates using previously described methods as follows: NADH dehydrogenase [ 5 ] , NADH cytochrome c reductase [ 5 ] , succinate dehydrogenase [ 6 ] , succinate cytochrome c reductase [ 6 ] , cytochrome c oxidase [ 7 ] , and citrate synthase [ 8 ] . Enzyme activities were expressed as μmol/min/g of wet weight tissue.
Glucose level (mg/dl [amount × 0.0555 = mmol/l]; measured 2 h post-load) was determined using the glucose oxidase method [24]; analyses using data on glucose levels excluded the five twin pairs for whom one or both twins had values 300 mg/dl or higher.
Loading...