Example sentences for: overnight

How can you use “overnight” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For ligand blot assays, samples separated by SDS-PAGE (9%) were transferred to PVDF overnight, blocked with 5% dried milk in TTBS (50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20, pH 7.5).

  • Briefly, this is an immunofluorescence assay in which 1:10 dilutions of the virus were prepared in shell vials and incubated at 37°C overnight.

  • The membrane was incubated overnight at 4°C on a shaker, washed 4 × 15 minutes with PBS at room temperature (RT), incubated with a horseradish peroxidase-coupled anti-mouse antibody (1:5000, Dianova, Hamburg) for 3 h at and then washed again 4 × 15 minutes with PBS at RT.

  • Aerate overnight.

  • Aggregates that formed blastocysts after overnight culture were allowed to continue their development in utero by transferring them to a pseudopregnant surrogate mother.


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