Words similar to ova

Example sentences for: ova

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For cytokines assays, 1 × 10 6transduced cells were incubated on anti-CD3 coated plates for 72 h at 37°C/5% CO 2 . Culture supernatants were then harvested and an ELISA assay was performed to measure the production of IL-4, IL-10 and IFN-γ as previously described [ 30 ] . To assay proliferation, the transduced cells were plated in duplicate in microtiter plates (5 × 10 5/well) at a ratio of 1:5 with irradiated BALB/c splenocytes and the indicated doses of OVA peptide at 37°C/5% CO 2 . After 72 h, 50 μCi/ml [ 3H]thymidine (Amersham, Piscataway, NJ) was added to the well.
The two exceptions were a single non-immunized mouse with anti-G7 IgG1 antibodies (at a serum concentration of 90 μg/ml), and one mouse immunized with HEL/OVA with anti-G7 IgG2a antibodies (at a serum concentration of 492 ng/ml).
10 TCR transgenic mice specific for ovalbumin peptide residues 323-339 (OVA323-339) and restricted by I-A d [ 20 ] were backcrossed onto BALB/cJ background (>16 generations).
However, the serum concentrations of total IgE were very similar in the mice that had been immunized with G7, PD or HEL/OVA peptides (Figure 3).
Similarly, while the group of mice that was challenged with G7 peptides exhibited a higher incidence of anaphylactic responses than did the group challenged with HEL/OVA peptides (Table 1), the temperature changes (Fig.