Example sentences for: ova

How can you use “ova” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Total IgE concentrations were slightly, but significantly, higher in the peptide-immunized groups (G7, PD, or HEL/OVA) compared to those in mice injected with IFA and saline alone (Figure 3).

  • The two exceptions were a single non-immunized mouse with anti-G7 IgG1 antibodies (at a serum concentration of 90 μg/ml), and one mouse immunized with HEL/OVA with anti-G7 IgG2a antibodies (at a serum concentration of 492 ng/ml).

  • injections of 100 μl containing a mixture of 200 μg each of the G7 peptides, the PD peptides or the HEL/OVA peptides, dissolved in 50 μl of sterile, pyrogen-free 0.9% NaCl ("saline") and emulsified in an equal volume of incomplete Freund's adjuvant (IFA) (Difco Laboratories, Detroit, MI).

  • For cytokines assays, 1 × 10 6transduced cells were incubated on anti-CD3 coated plates for 72 h at 37°C/5% CO 2 . Culture supernatants were then harvested and an ELISA assay was performed to measure the production of IL-4, IL-10 and IFN-γ as previously described [ 30 ] . To assay proliferation, the transduced cells were plated in duplicate in microtiter plates (5 × 10 5/well) at a ratio of 1:5 with irradiated BALB/c splenocytes and the indicated doses of OVA peptide at 37°C/5% CO 2 . After 72 h, 50 μCi/ml [ 3H]thymidine (Amersham, Piscataway, NJ) was added to the well.

  • For some experiments, the transduced cells were then activated with 5 μM ionomycin and 50 ng/ml PMA for 4 h or stimulated for 18 h by immobilized anti-CD3 (pre-coated at 10 μg/ml in PBS) or 5 μg/ml OVA peptide with antigen-presenting cells before analysis.

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