Example sentences for: out-of-frame

How can you use “out-of-frame” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • We mutated an out-of-frame ATG codon identified in a B1 template sequence during PCR amplification of the hook to avoid translational incompetence and resistance to 5-FOA.

  • This would result in an out-of-frame translation with the NIP2;1 -like coding sequence for 35 residues upstream of the position of the frameshift.

  • pARS-ALF contained two 40-bp fragments of alphoid DNA monomer that were PCR-amplified using the primer pair ALF-F and ALF-R from a 171-bp alphoid consensus sequence (X07685) [ 34 ] . None of the amplified targeting hooks contained an ATG codon that was out-of-frame in URA3 . Vectors pARS-TGAC and pARS-HPRT were linearized by Sal I, pARS-Alu by Sma I, and pARS-ALF by Mlu I, exposing the targeting sequences.

  • All those vectors were created with the Bluescript-based yeast- E. coli shuttle vector pRS313 (HIS3-CEN6-ARSH4) [ 32 ] . The cassette containing the URA3 gene driven by the Shizosaccharomyces pombe ADH1 promoter was derived from the pBL94 plasmid [ 19 ] . In the original cassette, the ADH1 promoter and URA3 were separated by a 35-bp adaptor containing an out-of-frame ATG initiation codon.

  • Both selected targeting sequences do not contain an ATG initiation codon that is out-of-frame with the vector URA3 , which avoids the translational incompetence of URA3 mRNA and resistance to 5-FOA (see Methods).

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