Words similar to orientation
Example sentences for: orientation
How can you use “orientation” in a sentence? Here are some example sentences to help you improve your vocabulary:
Orientation of transcripts was assessed by restricting which primers were present during RT single-strand synthesis.
Our initial GFP transposon,
, was created by placing the sequence encoding enhanced green fluorescent protein (EGFP) in one of these frames such that if the transposon landed in another coding sequence, in the correct orientation and frame, it would produce a GFP fusion protein (figure 1A). This fragment was excised with Not I and subcloned into the Not I site of the mammalian expression vector pcDNA3 (Invitrogen), in either the sense or antisense orientation.
Constructs in this vector were linearized at appropriate restriction sites to allow the sythesis of digoxigenin-UTP labeled complementary RNA in the antisense or sense orientation (using T3 or T7 RNA polymerase, respectively; Boehringer, Indianapolis, IN).
This includes a volunteer screening process, orientation, training and ongoing supervision and support by a professional staff.