Example sentences for: opti-mem

How can you use “opti-mem” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In brief, 2 × 10 8PBMC were thawed, washed twice in a 50:50 mixture of OPTI-MEM and Plasbumin (Bayer, West Heven, CT) and incubated for 3 h in a T175 flask in 50 ml serum-free OPTI-MEM in the presence or absence of 6,000 IU IL-2 per ml OPTI-MEM.

  • Supernatants obtained from PBMC exposed to IL-2 or OPTI-MEM (control) for 3 and 24 h were overlayed on confluent monolayers of HUVEC in T175 flasks (Falcon) at 37°C in 5% CO 2 for 3 h.

  • , washed twice in a 50:50 mixture of OPTI-MEM and Plasbumin and incubated for 3 and 24 h at 1 × 10 8cells/T175 flask in 50 ml serum-free OPTI-MEM in the presence or absence of 6,000 IU IL-2 per ml OPTI-MEM.

  • This mixture was diluted to 1 ml with Opti-mem and applied to cells in one well of 6-well tissue culture plates.

  • 2 ug of plasmid DNA and 25 ul of Lipofectamine were combined in 200 ul of Opti-mem media (Life Technologies) and allowed to stand at room temperature for 30 minutes.


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