Words similar to one-step

Example sentences for: one-step

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Deletion strains and epitope-tagged stains were constructed by one-step gene replacement using PCR-generated fragments containing kanamycin or ura4 cassettes [ 36 ] . Growth media, flow cytometry, and all other relevant S. pombe techniques were described previously [ 37 ] .
Nine independent gene-specific RT-PCR reactions were performed using the Superscript™ one-step RT-PCR kit according to the manufacturer (Invitrogen) and PCR products were cloned into the PCR2.
In earlier reports, Northern blot hybridization and RT-PCR failed to detect DFz1, DFz2, and DFz3 in S2 cells [ 6 17 18 ] . Using a one-step RT-PCR assay to measure the expression levels of all 4 DFzs and Arr in the same S2 cells used by Yanagawa et al . (Figure 1A), we detected DFz2, DFz3, and Arr mRNAs, but not DFz1 and DFz4 mRNAs after 40 cycles of amplification.
(The fraction of neutral mutants at distance d is equivalent to w ( d ) under the assumption that mutant sequences which fold into the same structure as the reference sequence have fitness 1, and all other mutants have fitness 0.) In addition, to distinguish neutral from compensatory mutations, we recorded at each Hamming distance d , for all viable sequences as well as a random sample of up to 1,000 non-viable sequences, the average number of viable sequences after an additional one-step mutation.
0.1 μg RNA from each cell line was used as a template for one-step RT-PCR (One-step RT-PCR kit from Invitrogen).