Example sentences for: one-step

How can you use “one-step” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The three transcripts selected to be investigated using quantitative one-step real time RT-PCR were OSU-T1-50, SAHH and HSC70.

  • Previous studies have shown that this choline is liberated from PC in a one-step reaction, thus indicating that it is produced by a phospholipase D (PLD, EC 3.1.

  • 0.1 μg RNA from each cell line was used as a template for one-step RT-PCR (One-step RT-PCR kit from Invitrogen).

  • Deletion strains and epitope-tagged strains were constructed by one-step gene replacement using PCR-generated fragments containing kanamycin or ura4 cassettes [ 32].

  • Nine independent gene-specific RT-PCR reactions were performed using the Superscript™ one-step RT-PCR kit according to the manufacturer (Invitrogen) and PCR products were cloned into the PCR2.


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