Words similar to one-step

Example sentences for: one-step

How can you use “one-step” in a sentence? Here are some example sentences to help you improve your vocabulary:

(The fraction of neutral mutants at distance d is equivalent to w ( d ) under the assumption that mutant sequences which fold into the same structure as the reference sequence have fitness 1, and all other mutants have fitness 0.) In addition, to distinguish neutral from compensatory mutations, we recorded at each Hamming distance d , for all viable sequences as well as a random sample of up to 1,000 non-viable sequences, the average number of viable sequences after an additional one-step mutation.
Deletion strains and epitope-tagged strains were constructed by one-step gene replacement using PCR-generated fragments containing kanamycin or ura4 cassettes [ 32].
0.1 μg RNA from each cell line was used as a template for one-step RT-PCR (One-step RT-PCR kit from Invitrogen).
Deletion strains and epitope-tagged stains were constructed by one-step gene replacement using PCR-generated fragments containing kanamycin or ura4 cassettes [ 36 ] . Growth media, flow cytometry, and all other relevant S. pombe techniques were described previously [ 37 ] .
The RT-PCR were performed in the same sample tube using gene-specific primers obtained from IDT (Coralville, IA), as described below, and the Superscript one-step kit obtained from Gibco-BRL (Grand Island, NY).