Example sentences for: one-step

How can you use “one-step” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 0.1 μg RNA from each cell line was used as a template for one-step RT-PCR (One-step RT-PCR kit from Invitrogen).

  • The RT-PCR were performed in the same sample tube using gene-specific primers obtained from IDT (Coralville, IA), as described below, and the Superscript one-step kit obtained from Gibco-BRL (Grand Island, NY).

  • The three transcripts selected to be investigated using quantitative one-step real time RT-PCR were OSU-T1-50, SAHH and HSC70.

  • Deletion strains and epitope-tagged stains were constructed by one-step gene replacement using PCR-generated fragments containing kanamycin or ura4 cassettes [ 36 ] . Growth media, flow cytometry, and all other relevant S. pombe techniques were described previously [ 37 ] .

  • Deletion strains and epitope-tagged strains were constructed by one-step gene replacement using PCR-generated fragments containing kanamycin or ura4 cassettes [ 32].


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