Example sentences for: one-step

How can you use “one-step” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Deletion strains and epitope-tagged strains were constructed by one-step gene replacement using PCR-generated fragments containing kanamycin or ura4 cassettes [ 32].

  • (The fraction of neutral mutants at distance d is equivalent to w ( d ) under the assumption that mutant sequences which fold into the same structure as the reference sequence have fitness 1, and all other mutants have fitness 0.) In addition, to distinguish neutral from compensatory mutations, we recorded at each Hamming distance d , for all viable sequences as well as a random sample of up to 1,000 non-viable sequences, the average number of viable sequences after an additional one-step mutation.

  • The three transcripts selected to be investigated using quantitative one-step real time RT-PCR were OSU-T1-50, SAHH and HSC70.

  • Previous studies have shown that this choline is liberated from PC in a one-step reaction, thus indicating that it is produced by a phospholipase D (PLD, EC 3.1.

  • Deletion strains and epitope-tagged stains were constructed by one-step gene replacement using PCR-generated fragments containing kanamycin or ura4 cassettes [ 36 ] . Growth media, flow cytometry, and all other relevant S. pombe techniques were described previously [ 37 ] .


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