Words similar to one-step

Example sentences for: one-step

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Deletion strains and epitope-tagged strains were constructed by one-step gene replacement using PCR-generated fragments containing kanamycin or ura4 cassettes [ 32].
The RT-PCR were performed in the same sample tube using gene-specific primers obtained from IDT (Coralville, IA), as described below, and the Superscript one-step kit obtained from Gibco-BRL (Grand Island, NY).
Deletion strains and epitope-tagged stains were constructed by one-step gene replacement using PCR-generated fragments containing kanamycin or ura4 cassettes [ 36 ] . Growth media, flow cytometry, and all other relevant S. pombe techniques were described previously [ 37 ] .
In earlier reports, Northern blot hybridization and RT-PCR failed to detect DFz1, DFz2, and DFz3 in S2 cells [ 6 17 18 ] . Using a one-step RT-PCR assay to measure the expression levels of all 4 DFzs and Arr in the same S2 cells used by Yanagawa et al . (Figure 1A), we detected DFz2, DFz3, and Arr mRNAs, but not DFz1 and DFz4 mRNAs after 40 cycles of amplification.
0.1 μg RNA from each cell line was used as a template for one-step RT-PCR (One-step RT-PCR kit from Invitrogen).