Words similar to one-step

Example sentences for: one-step

How can you use “one-step” in a sentence? Here are some example sentences to help you improve your vocabulary:

The three transcripts selected to be investigated using quantitative one-step real time RT-PCR were OSU-T1-50, SAHH and HSC70.
Previous studies have shown that this choline is liberated from PC in a one-step reaction, thus indicating that it is produced by a phospholipase D (PLD, EC 3.1.
In earlier reports, Northern blot hybridization and RT-PCR failed to detect DFz1, DFz2, and DFz3 in S2 cells [ 6 17 18 ] . Using a one-step RT-PCR assay to measure the expression levels of all 4 DFzs and Arr in the same S2 cells used by Yanagawa et al . (Figure 1A), we detected DFz2, DFz3, and Arr mRNAs, but not DFz1 and DFz4 mRNAs after 40 cycles of amplification.
Deletion strains and epitope-tagged strains were constructed by one-step gene replacement using PCR-generated fragments containing kanamycin or ura4 cassettes [ 32].
0.1 μg RNA from each cell line was used as a template for one-step RT-PCR (One-step RT-PCR kit from Invitrogen).