Words similar to omp
Example sentences for: omp
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Similarly, the epitopes recognized by monoclonal antibodies to outer membrane protein P5, an OMP A-like protein, and P6, a highly conserved protein in all strains of nontypeable H. influenzae , were expressed in the outer membranes of cells grown as biofilms and cells grown planktonically (Figure 4).
Published methods include enterobacterial repetitive intergenic consensus sequence-PCR (ERIC-PCR), and repetitive intergenic palindromic sequence-PCR (REP-PCR) [ 2 3 ] ; random amplified polymorphic DNA-PCR (RAPD-PCR) or arbitrary primed-PCR (AP-PCR) [ 4 5 ] ; and restriction fragment length polymorphism-PCR (RFLP-PCR) of the omp 2 locus [ 6 7 ] . ERIC-PCR and RAPD-PCR are both affected by assay conditions and environmental effects during the amplification process [ 8 9 10 ] . Although the results are highly reproducible within a laboratory, laboratory-to-laboratory reproducibility has been problematic and thus makes the universal application of these methods unlikely (compare [ 2 ] and [ 3 ] ). RFLP-PCR of the omp 2 locus is not constrained by issues of reproducibility and has been useful for the differentiation of Brucella species [ 6 ] and for differentiation among isolates from marine mammal hosts [ 7 ] . However, as a tool for the epidemiology of brucellosis in livestock, this technique is limited by the low rate of natural sequence divergence within the locus at the biovar level.
The outer membrane proteins prepared from cells grown in the absence of iron bound significantly higher amount of radioactive iron siderophore complex as compared to that of the OMP prepared from cells grown in iron rich conditions (p value < 0.05).
This strongly indicates that OMP of CDM-Fe grown bacteria contains the IROMPs and that they bind to the iron siderophore complex, hence they may be the receptor for siderophore iron complex.
To determine the nature of the binding receptor, the OMP preparation was subjected to digestion with proteinase K (10 mg/ml) for 1 hr at 37°C prior to the incubation with iron siderophore complex.
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