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Example sentences for: offee

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Multiple alignments were constructed using the T-Coffee program [ 32], followed by manual correction based on the PSI-BLAST results.
The overall alignment of the midasin M-domain from humans, yeast and Giardia with other MIDAS-containing proteins (three Mg-chelatases and the I-domains of three integrins) was created by first aligning the midasins, Mg-chelatases and integrins separately with the program T-Coffee [ 58 ] . The resultant three partial alignments were then combined by the profile alignment procedure of ClustalX, as above.
Multiple sequence alignments were prepared for the individual clusters using T-Coffee [ 35] and their secondary structure was predicted with the PHD program [ 36].
Sets of orthologous ribosomal proteins were extracted from the COG database, and their amino acid sequences were aligned using the T-Coffee program [ 45 ] , with subsequent manual validation and removal of poorly aligned regions.
A multiple alignment of the RDRPs and YRHs was constructed using the T-Coffee program and adjusted using the alignments reported by PSI-BLAST and secondary structure prediction.