Words similar to nylon
Example sentences for: nylon
How can you use “nylon” in a sentence? Here are some example sentences to help you improve your vocabulary:
Following electrophoresis, RNA was transferred to a nylon membrane (Hybond-N+, Millipore, Bedford, MA) according to the manufacturer's instructions, and hybridized with digoxigen-dUTP-labeled probe as described previously [ 4 ] . The primers used to generate the probe were: 5'-GGAATTCACCTGCTAATTACCTGA-3'and 5'-CGCGGATCCGTTTACACATAGTTATTGATAGAATCT-3'
The cells were briefly hemolyzed, washed extensively in DMEM containing 10% FBS, filtered through a 100 μM sterile nylon filter, counted by hemacytometer, and suspended in complete medium.
Alternatively, cDNA can be spotted on nylon filters or glass slides.
An overview of the experimental design is presented in Table 1, and an example of a microarray image generated by hybridisation of pig skeletal muscle RNA to a human cDNA nylon microarray is shown in Figure 1. The complete dataset is provided as an additional file, see Additional file 1: [data.xls].
For northern blotting, 20 μg of total RNA was electrophorized on a denaturing agarose gel, transferred to nylon membrane, and hybridized to 33P labeled cDNA probes as described previously [ 11 ] .
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