Example sentences for: nylon

How can you use “nylon” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Following electrophoresis, RNA was transferred to a nylon membrane (Hybond-N+, Millipore, Bedford, MA) according to the manufacturer's instructions, and hybridized with digoxigen-dUTP-labeled probe as described previously [ 4 ] . The primers used to generate the probe were: 5'-GGAATTCACCTGCTAATTACCTGA-3'and 5'-CGCGGATCCGTTTACACATAGTTATTGATAGAATCT-3'

  • The cells were briefly hemolyzed, washed extensively in DMEM containing 10% FBS, filtered through a 100 μM sterile nylon filter, counted by hemacytometer, and suspended in complete medium.

  • Alternatively, cDNA can be spotted on nylon filters or glass slides.

  • An overview of the experimental design is presented in Table 1, and an example of a microarray image generated by hybridisation of pig skeletal muscle RNA to a human cDNA nylon microarray is shown in Figure 1. The complete dataset is provided as an additional file, see Additional file 1: [data.xls].

  • For northern blotting, 20 μg of total RNA was electrophorized on a denaturing agarose gel, transferred to nylon membrane, and hybridized to 33P labeled cDNA probes as described previously [ 11 ] .


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