Words similar to ny
Example sentences for: ny
How can you use “ny” in a sentence? Here are some example sentences to help you improve your vocabulary:
After 15 min on ice, the lysates were sonicated using a SonicatorTM Cell Disruptor (Heat Systems-Ultrasonic, Inc., Plainview, NY) for 5 seconds, and centrifuged at 11000 × g for 20 min at 4°C.
Total RNA (20 μg) was used to synthesize double-stranded cDNA using Superscript ®Choice System (Life Technologies), and the template for an in vitro transcription reaction was used to synthesize biotin-labeled antisense cRNA (BioArray™ High Yield RNA Transcript Labeling Kit; Enzo Diagnostics, Farmingdale, NY, USA).
Cells were grown in DMEM medium containing 10% FBS, 100 units/ml penicillin/streptomycin for nine hours in 5% CO 2 and transfected with 10 μg mouse 5-HT 3AS R cDNA per 100 mm dish using the calcium phosphate technique (New Life Technologies, NY).
To detect the expressed myc-tagged Rab proteins, cells were incubated for 60 min with either the 9E10 mouse monoclonal antibody against the myc epitope or a rabbit polyclonal antibody against the same epitope (Upstate Biotechnology, Lake Placid, NY).
Total cellular RNA was isolated as described previously [ 11 ] and 1.2 - 5 μg was used for reverse transcription with 5'-T 30 NN-3' primer using Superscript II Rnase H-RT according to the manufacturer's specifications (Life Technologies, Grand Island, NY).