Words similar to ntps
Example sentences for: ntps
How can you use “ntps” in a sentence? Here are some example sentences to help you improve your vocabulary:
The purified PCR product (5 μl) was transcribed in 96-well format by incubation for 2 h at 37°C after the addition of 5 μl of a polymerase reaction cocktail consisting of 2 U T7 RNA polymerase, 4.6 U RNase inhibitor, 10 mM NTPs, 3.5 mM digoxigenin-11-UTP, 40 mM Tris pH 8.0, 6 mM MgCl 2 , 10 mM DTT and 2 mM spermidine.
After hybridization, scatter plots of hybridization intensities were generated comparing those intensities generated from unmodified cRNA (control) to either the duplicate control hybridization or to intensities generated from cRNA where one of the modified NTPs was incorporated.
Because the sensitivity and reproducibility of microarrays depends on the specific activity of target cRNA, we also wanted to determine if adding modified NTPs to the IVT changed the incorporation rate of biotin-11-UTP.
As seen in Figure 1A, when only the unmodified NTPs are incorporated during the IVT, there is good separation of the individual nucleosides.
The effect of modified NTPs on differential expression ratios
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