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Example sentences for: novagen

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3, a protein band of approximately 37,000 Mr was produced from the pET-15b-PfPP1 clone, which is in agreement with the predicted molecular weight of 34,904 of PfPP1 and roughly another 2,000 added for the (His) 6 region of pET-15b (Novagen: Madison, WI).
Introduction of the plasmids into E. coli BL21(DE3) cells containing the compatible RIG plasmid [ 39 ] , induction of the proteins with IPTG, and purification through Ni-chelation chromatography were performed using the His-Bind kit reagents and procedure (Novagen).
Cells were fixed and processed for immunofluorescence as described [ 4 20 ] . The N-terminal fragment expressed by fs-α 1S or wt-α 1S was identified with a mouse monoclonal antibody against a T7 epitope fused to the N-terminus of α 1S . The anti-T7 antibody (Novagen, WI) was used at a dilution of 1:1000.
For Subject #4 where the slides contained sections from the vas deferens and seminal vesicle (included by Novagen, the commercial supplier), the epithelia of these tissues were also immuno-stained, indicating the presence of SPAM1 (data not shown).
E. coli HMS174 (DE3) (Novagen, Madison, WI) was used for the genetic mobility assay.