Words similar to novagen

Example sentences for: novagen

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The wild type autogene thus obtained (pET/T7p/T7) was transformed into strain HMS174 pLysS (Novagen, Madison, WI), the same cell line Studier et al.
The latter indicates that SPAM1 is associated with the principal cells of the epithelium, consistent with the localization of the transcript and with findings in the mouse [ 11 12 ] . The finding of human SPAM1 in the seminal vesicle, which was examined only because it was included in the tissue series on the slides from Novagen, was unexpected but not surprising.
Recombinant V80R and A61D mutants were expressed in E. Coli BL21 (DE3) pLysS cells (Novagen), and purified by Ni-NTA (Qiagen) and HiTrap SP (Pharmacia) affinity column chromatography followed by ammonium sulfate precipitation as described by Kim et al [ 7 ] .
PMSF, DTT, BSA and protease inhibitor cocktail (Novagen) were added to 0.1 mM, 1 mM, 2μg/ml and 1/1000 dilution respectively.
2. 3) A 1903-bp fragment containing oriM was recovered from pMV261 by digestion with Not I and Mlu I, gel purified, blunt ended using the Perfectly Blunt Cloning Kit (Novagen), and ligated into pSE.