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Example sentences for: novagen

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The gel-purified PCR products were cloned into either a pSTBlue-1 vector (Novagen, Madison, WI) or pCR 4-TOPO TA vector (Invitrogen, Carlsbad, CA), according to the manufacturer's protocol.
Transformation of DNA into E. coli was accomplished by using commercially obtained competent cells (Novagen, Madison, WI).
1 (TA cloning kit, Invitrogen, Carlsbad, CA) and then into plasmid pET28a+ (Novagen, Madison, WI), which contained the wild-type T7 RNA polymerase promoter.
Cells were fixed and processed for immunofluorescence as described [ 4 20 ] . The N-terminal fragment expressed by fs-α 1S or wt-α 1S was identified with a mouse monoclonal antibody against a T7 epitope fused to the N-terminus of α 1S . The anti-T7 antibody (Novagen, WI) was used at a dilution of 1:1000.
After clarification by centrifugation at 12,000 × g for 10 min, the cell extract was incubated with T7 tag antibody (Ab)-agarose beads (Novagen) for 2 h.