Example sentences for: novagen

How can you use “novagen” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To assemble Net1N beads with Cdc14 for six reactions (Figure 4A, detection method: Coomassie Blue staining), 105 μl of anti-T7 beads (Novagen) were incubated with 24 μg of His6-T7-Net1N in 100 μl of HEBD for 1 hr, washed four times with HBS + 0.2% n-octylglucoside + 2 mM DTT, and incubated with 6 μg of GST-Cdc14 in 100 μl of HEBD for 1 hr.

  • Protein induction was performed using isopropyl-β-D-thiogalactopyranoside (IPTG) as described by Novagen Inc.

  • The wild type autogene thus obtained (pET/T7p/T7) was transformed into strain HMS174 pLysS (Novagen, Madison, WI), the same cell line Studier et al.

  • We used the Quickchange kit (Stratagene) to generate site directed mutants, V80R and A61D from wild-type TEL-SAM cloned into a modified pET3c vector (Novagen) [ 7 ] . The expressed protein sequence includes an MEKTR leader sequence, followed by residues 38-124 of the TEL protein and then a C-terminal His tag.

  • Wax-embedded embryonic sections were purchased from Novagen (Madison, Wl).


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