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Example sentences for: novagen

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The PCR product was then cloned into the pET15b vector which carries an N-terminal Histag sequence followed by BamH1 and Nde 1 cloning sites (Novagen Inc., Madison, WI).
Transformation of DNA into E. coli was accomplished by using commercially obtained competent cells (Novagen, Madison, WI).
For in vitro binding studies, about 5 μg of (His) 6 P protein was bound to 25 μl (packed volume) of Ni +2-charged NTA column using the binding buffer provided by the manufacturer (Novagen, Madison, WI).
2. 3) A 1903-bp fragment containing oriM was recovered from pMV261 by digestion with Not I and Mlu I, gel purified, blunt ended using the Perfectly Blunt Cloning Kit (Novagen), and ligated into pSE.
After clarification by centrifugation at 12,000 × g for 10 min, the cell extract was incubated with T7 tag antibody (Ab)-agarose beads (Novagen) for 2 h.