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Example sentences for: novagen

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Transformation of DNA into E. coli was accomplished by using commercially obtained competent cells (Novagen, Madison, WI).
Recombinant V80R and A61D mutants were expressed in E. Coli BL21 (DE3) pLysS cells (Novagen), and purified by Ni-NTA (Qiagen) and HiTrap SP (Pharmacia) affinity column chromatography followed by ammonium sulfate precipitation as described by Kim et al [ 7 ] .
For in vitro binding studies, about 5 μg of (His) 6 P protein was bound to 25 μl (packed volume) of Ni +2-charged NTA column using the binding buffer provided by the manufacturer (Novagen, Madison, WI).
1 (TA cloning kit, Invitrogen, Carlsbad, CA) and then into plasmid pET28a+ (Novagen, Madison, WI), which contained the wild-type T7 RNA polymerase promoter.
The latter indicates that SPAM1 is associated with the principal cells of the epithelium, consistent with the localization of the transcript and with findings in the mouse [ 11 12 ] . The finding of human SPAM1 in the seminal vesicle, which was examined only because it was included in the tissue series on the slides from Novagen, was unexpected but not surprising.