Words similar to noti

Example sentences for: noti

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A XbaI/NotI fragment from clone 4B1 and a NotI fragment from 6A1 hybridized to the probe and were subcloned in pBluesript II SK(-) (Stratagene) for further analysis.
In comparison, the labelling of the Bgl I was less affected by artifacts resulting from labelling of internal restriction sites within the cDNA as compared to labeling of a NotI site in a different vector construct, although Bgl I sites are more often found in cDNAs compared to Not I sites (data not shown).
A potential translation initiation codon in the NotI/BamHI fragment (3 kb) of clone 4B1 starts an open reading frame that encodes a polypeptide sequence highly homologous to the first six transmembrane domains of the human EP2 receptor [ 20 ] . The amino acid sequence of the open reading frame in the NotI fragment (6 kb) of clone 6A was homologous to the seventh transmembrane domain (TMD VII), followed by a short C-terminal tail and an in-frame stop codon.
This was inserted into BigT digested with XhoI, Klenow filled, and then digested with NotI.
The resulting construct was further digested with NotI and PstI and combined with a NotI-PstI fragment containing the remaining c-terminal portion of a previously described MmCOP1 cDNA clone (GenBank accession number AF151110.