Example sentences for: noti

How can you use “noti” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A NotI (blunt) 3.6 kb lacZ fragment from pCMV-βgal (Clontech) was ligated into this SalI (blunt) site to generate a targeting vector that could be used to introduce lacZ into the "marked" Hnf3 α locus.

  • This was inserted into BigT digested with XhoI, Klenow filled, and then digested with NotI.

  • 7) were generated by appending C/EBPα fragments to the NotI site of DBD + 1-154, or to the SgrAI site of DBD.

  • The DBD and DBD + 154-257 derivatives of C/EBPα were constructed by replacing, respectively, the amino terminal 257 amino acids of C/EBPα-GFP (to the SgrAI site of C/EBPα) and the amino terminal 153 amino acids of C/EBPα-GFP (to the NotI site of C/EBPα) with oligonucleotides containing a strong Kozak sequence.

  • DBD + 1-154 was constructed by replacing amino acids 155 to 257 (from the NotI to SgrAI sites) with an oligonucleotide.


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