Words similar to noti
Example sentences for: noti
How can you use “noti” in a sentence? Here are some example sentences to help you improve your vocabulary:
The PCR product was cloned into the NotI and SalI sites in PMI-hCD2 [ 46 ] . The coding sequence for the Rfz-2/β 2 AR chimeric receptor in CS2+ [ 6 37 ] was digested with EcoRI and XhoI and cloned into PMI-hCD2 at the EcoRI and SalI sites.
The resulting construct was further digested with NotI and PstI and combined with a NotI-PstI fragment containing the remaining c-terminal portion of a previously described MmCOP1 cDNA clone (GenBank accession number AF151110.
A NotI (blunt) 3.6 kb lacZ fragment from pCMV-βgal (Clontech) was ligated into this SalI (blunt) site to generate a targeting vector that could be used to introduce lacZ into the "marked" Hnf3 α locus.
A MCS containing the restriction sites for XhoI, ApaI, SacII, NotI, SacI, EcoRV, and KpnI was synthesized and inserted downstream of the second loxP site by digesting the DNA with XhoI and KpnI and ligating in the MCS, which had been synthesized to have compatible cohesive ends.
To make the R26R-CFP targeting construct pECFP was digested with AgeI, Klenow filled then digested with NotI to excise the ECFP cDNA.