Example sentences for: nonidet

How can you use “nonidet” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A solution of 10% Nonidet P-40 was added to a final concentration of 0.025%.

  • The cells were resuspended in buffer containing 50 mM Tris-HCl (pH 7.5), 1% Nonidet P-40, and 150 mM NaCl.

  • MgSO 4 , (ethylene-bis [oxyethylenenitrilo])tetraacetic acid (EGTA), GTP (type II-S), glycerol, 2-( N -morpholino)-ethane sulfonic acid (MES), Nonidet P 40, piperazine- N,N '-bis(2-ethanesulfonic acid) (PIPES) and Triton X-100 were purchased from Sigma Chemical Company.

  • Nuclear extracts were obtained as described elsewhere [ 19 ] . Briefly, detached cells were resuspended in buffer A (10 mM Hepes pH 7.9, 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1 mM dithiothreitol, 0.5 mM phenylmethylsulfonyl fluoride) and cells were lysed with Nonidet P-40.

  • Whole cell lysates were made from GC monolayers by standard techniques using a lysis buffer consisting of PBS with 1% Nonidet P40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor cocktail (1:100, Sigma P8340).


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