Words similar to nonidet

Example sentences for: nonidet

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To prepare cell lysates, freshly isolated trophoblast cells and early (16-24 h) and late trophoblast cultures (72-96 h) were washed twice with ice-cold PBS, lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5, 200 mM NaCl) containing 1 mM sodium orthovanadate, 0.25% Nonidet P-40, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride, sonicated, centrifuged, and supernatants stored at -80°C until use [ 15 ] .
Whole cell lysates were made from GC monolayers by standard techniques using a lysis buffer consisting of PBS with 1% Nonidet P40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor cocktail (1:100, Sigma P8340).
The labelled probe molecules were detected with rhodamine anti-digoxigenin (Oncor, Inc., Gaithersburg, MD) and the slides were washed 3 times in PBS and 0.1 % Nonidet NP-40.
Cells were lysed for 15 min at 4°C in EBC buffer (50 mM Tris-HCl, pH 8.0, 120 mM NaCl, 0.5% Nonidet P-40) containing 10 ug/ml of the protease inhibitors aprotinin, leupeptin, and phenylmethylsulfonylfluoride (PMSF).
Cultures were lysed in 50 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM EDTA, 2.5 mM EGTA, 1 mM DTT, 1% Nonidet P-40, 10% glycerol, 1 mM NaF, 0.1 mM sodium orthovanadate, and protease inhibitors for 30 minutes at 4°C.