Words similar to nonidet
Example sentences for: nonidet
How can you use “nonidet” in a sentence? Here are some example sentences to help you improve your vocabulary:
To prepare cell lysates, freshly isolated trophoblast cells and early (16-24 h) and late trophoblast cultures (72-96 h) were washed twice with ice-cold PBS, lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5, 200 mM NaCl) containing 1 mM sodium orthovanadate, 0.25% Nonidet P-40, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride, sonicated, centrifuged, and supernatants stored at -80°C until use [ 15 ] .
15 (50 mM HEPES/KOH, pH 7.5, 150 mM KCl, 0.02% Nonidet P-40, 5 mM magnesium acetate, 1 mM EDTA, 1 mM EGTA, 10% glycerol, 1 mM phenylmethylsulfonyl fluoride, 2 μg/ml pepstatin, 2 μg/ml leupeptin, 5 μg/ml aprotinin, 1 mM dithiothreitol).
For preparation of protein lysates from tissue samples (levator ani muscle; rectus fascia = ~negative control; and whole placenta = low p27 positive control) 100 tissue sections (7 μm thick) were collected into microcentrifuge tubes and lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5,200 mM NaCI, 0.25% Nonidet P-40) containing 1 mM sodium orthovanadate, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride (400 μl/100 mg of tissue sections).
Cells were lysed for 15 min at 4°C in EBC buffer (50 mM Tris-HCl, pH 8.0, 120 mM NaCl, 0.5% Nonidet P-40) containing 10 ug/ml of the protease inhibitors aprotinin, leupeptin, and phenylmethylsulfonylfluoride (PMSF).
Subsequently, the wells were washed 10 × with ice cold PBS or 1:50 anti-p24 mAb in Ch-PBS for HIV-1 BaL, and then treated with lysis buffer (1% Nonidet P40 [NP40], 100 μg/ml BSA in PBS) for 30 min at 37°C.
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