Example sentences for: nonfat

How can you use “nonfat” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The blots were preincubated for 30 minutes at 22°C in Tris-buffered saline containing 5% nonfat powdered milk and 0.2% Tween-20 (TBS-T), and were then probed with affinity purified anti-AQP1 IgG (1:5000) for 2 hr at 22°C.

  • The nonspecific binding sites on the plate were blocked by 2% nonfat milk powder at 37°C for 3 hrs. 100 μl of the antibody was added to the wells and the plates were further incubated for 3 hrs at 37°C.

  • 5 mM Tris, pH 6.7) at 50°C for 30 min, washed 4 times with TBS-Tween, blocked with 5% nonfat dry milk in TBS-Tween, then probed with anti-p44/42 ERK antibody according to the manufacturer's recommendations.

  • Following electrophoresis, the gel contents were electrophoretically-transferred to nitrocellulose (500 mA, 60-90 V, 1 hour, 4°C, Transfer Buffer II from Harlow and Lane [ 50 ] ). The membrane was blocked with 5% w/v nonfat dried milk in PBS-0.

  • The membrane was blocked with 5% nonfat milk in 50 M Tris-HCl/150 M NaCl, pH 7.5, and sequentially treated with affinity-purified rabbit antisera raised against recombinant mactinin, followed by second antibody conjugated with alkaline phosphatase (ICN, Costa Mesa, CA).


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