Words similar to nonfat
Example sentences for: nonfat
How can you use “nonfat” in a sentence? Here are some example sentences to help you improve your vocabulary:
Non-specific binding sites were blocked by incubating the nitrocellulose for 60 minutes in blocking buffer (25 mM Tris-HCl/Base, 150 mM NaCl, 0.05% Tween-20, and 3% nonfat dry milk).
Cells were washed with PBS and then incubated in blocking buffer consisting of TBS (50 mM Tris, pH 7.5, 150 mM NaCl) with 1% Triton X-100 and 2.5% nonfat milk.
Proteins were transferred onto Immobilon P membrane (Millipore), blocked in 5 % nonfat dry milk dissolved in tris-buffered saline / 0.1 % Tween and probed with the various antibodies.
After three washes with 0.1% Tween/TBS, the membranes were incubated for 1 h at room temperature with the appropriate horseradish peroxidase conjugated secondary antibody diluted 1:1500 in 5% nonfat milk/0.
Duplicate unknown tissue samples were prepared by combining them with known levels of PC-tubulin (1 : 1), then mixing them 1 : 1 with antibody in 3% nonfat dried milk in PBS.