Example sentences for: nonfat

How can you use “nonfat” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Samples were separated on 4-20% gradient Novex gels, transferred to Immobilon P membranes, blocked with 5% nonfat dry milk in TBS-Tween, then probed with anti-phospho-p44/42 MAPK antibody according to the manufacturer's recommendations.

  • Cells were washed with PBS and then incubated in blocking buffer consisting of TBS (50 mM Tris, pH 7.5, 150 mM NaCl) with 1% Triton X-100 and 2.5% nonfat milk.

  • After three washes with 0.1% Tween/TBS, the membranes were incubated for 1 h at room temperature with the appropriate horseradish peroxidase conjugated secondary antibody diluted 1:1500 in 5% nonfat milk/0.

  • The filters were blocked with 2% nonfat dry milk in 10 mM Tris-HCl (pH 8.0), 0.15 M sodium chloride, 0.2% Tween-20 (TBST) then incubated with primary antibody (1:1000 dilution) for 2 hours at room temperature.

  • The FDA currently regulates that nonfat delivery device, olestra.)


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