Words similar to nonfat
Example sentences for: nonfat
How can you use “nonfat” in a sentence? Here are some example sentences to help you improve your vocabulary:
Blots were incubated overnight with anti-Tie1 and anti-Tie2 antibodies (R&D Systems) at 1:5000, in Tris-buffered saline Tween containing 5% nonfat milk.
To detect intracellular TTP, fixed cells were washed twice in permeabilization buffer (consisting of staining buffer with the addition of 1 μg/ml saponin), centrifuged as before, and incubated for 30 min at 4°C in blocking buffer, consisting of 3% IgG with 3% nonfat powdered milk for leukocytes in permeabilization buffer.
Duplicate standard curves for each antibody were made with PC-tubulin (final concentration 150 μg/ml, 3% nonfat dried milk in PBS).
The membrane was blocked with 5% nonfat milk in 50 M Tris-HCl/150 M NaCl, pH 7.5, and sequentially treated with affinity-purified rabbit antisera raised against recombinant mactinin, followed by second antibody conjugated with alkaline phosphatase (ICN, Costa Mesa, CA).
The membranes were blocked in TBST (10 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.1% Tween-20) with 5% nonfat dry milk (w/v).