Example sentences for: nonfat

How can you use “nonfat” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The membranes were blocked with 5% nonfat milk and incubated at room temperature for 1 hour with anti-TGF-β1 polyclonal antibody at a 1:200 dilution, anti-TGF-β3 polyclonal antibody at a 1:1000 dilution, and anti Bcl-xS polyclonal antibody at 1:500 dilution.

  • Non-specific binding sites were blocked by incubating the nitrocellulose for 60 minutes in blocking buffer (25 mM Tris-HCl/Base, 150 mM NaCl, 0.05% Tween-20, and 3% nonfat dry milk).

  • Following electrophoresis, the gel contents were electrophoretically-transferred to nitrocellulose (500 mA, 60-90 V, 1 hour, 4°C, Transfer Buffer II from Harlow and Lane [ 50 ] ). The membrane was blocked with 5% w/v nonfat dried milk in PBS-0.

  • Cells were washed with PBS and then incubated in blocking buffer consisting of TBS (50 mM Tris, pH 7.5, 150 mM NaCl) with 1% Triton X-100 and 2.5% nonfat milk.

  • The membranes were blocked in TBST (10 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.1% Tween-20) with 5% nonfat dry milk (w/v).


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