Words similar to nonfat

Example sentences for: nonfat

How can you use “nonfat” in a sentence? Here are some example sentences to help you improve your vocabulary:

The membranes were blocked for 1 h at room temperature with 5% nonfat milk in Tris-buffered saline (TBS: 100 mM Tris, 0.9% NaCl, pH 7.5) containing 0.1% Tween 20, then incubated overnight at 4°C with primary antibody diluted in the blocking solution.
Proteins were transferred onto Immobilon P membrane (Millipore), blocked in 5 % nonfat dry milk dissolved in tris-buffered saline / 0.1 % Tween and probed with the various antibodies.
Cells were washed with PBS and then incubated in blocking buffer consisting of TBS (50 mM Tris, pH 7.5, 150 mM NaCl) with 1% Triton X-100 and 2.5% nonfat milk.
5 mM Tris, pH 6.7) at 50°C for 30 min, washed 4 times with TBS-Tween, blocked with 5% nonfat dry milk in TBS-Tween, then probed with anti-p44/42 ERK antibody according to the manufacturer's recommendations.
Plates were left at 22°C under mild humidity for 2 hours on a shaker and then washed four times with PBS and blotted dry before addition of the secondary horseradish peroxidase-conjugated antibody (Sigma) in 3% nonfat dried milk in PBS.