Example sentences for: nonfat

How can you use “nonfat” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The membranes were blocked with 5% nonfat milk and incubated at room temperature for 1 hour with anti-TGF-β1 polyclonal antibody at a 1:200 dilution, anti-TGF-β3 polyclonal antibody at a 1:1000 dilution, and anti Bcl-xS polyclonal antibody at 1:500 dilution.

  • Nonspecific binding sites were blocked by incubating the NC paper in 2% bovine serum albumin and 3% nonfat milk powder at 37°C for 3 hours.

  • Plates were left at 22°C under mild humidity for 2 hours on a shaker and then washed four times with PBS and blotted dry before addition of the secondary horseradish peroxidase-conjugated antibody (Sigma) in 3% nonfat dried milk in PBS.

  • To detect intracellular TTP, fixed cells were washed twice in permeabilization buffer (consisting of staining buffer with the addition of 1 μg/ml saponin), centrifuged as before, and incubated for 30 min at 4°C in blocking buffer, consisting of 3% IgG with 3% nonfat powdered milk for leukocytes in permeabilization buffer.

  • After three washes with 0.1% Tween/TBS, the membranes were incubated for 1 h at room temperature with the appropriate horseradish peroxidase conjugated secondary antibody diluted 1:1500 in 5% nonfat milk/0.


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