Words similar to nonessential
Example sentences for: nonessential
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Another development that has in the past served as an immediate warning of U.S. use of force: the withdrawal of nonessential State Dept. personnel and their dependents from embassies (in Israel and Kuwait), and of U.N. inspectors and humanitarian aid workers from Iraq.
Culture medium was prepared with low-glucose Dulbecco's modified Eagle medium with 10mmol/l HEPES (GIBCO BRL, Grand Island, NY, USA), 0.1mmol/l nonessential amino acids (Sigma Chemical, St. Louis, MO, USA), 0.4 mmol/l L-proline (Sigma Chemical), and 1mmol/l gadolinium-DTPA (Magnevist, Berlex Imaging, Wayne, NJ, USA).
Third, we grew 54 independent liquid cultures of the heterozygotes in nonselective medium and then selected for cells that were Arg +and Ura +. Uracil prototrophy requires restitution of the integrity of the disrupted ura3 gene of the UAU1 cassette by recombination between the overlapping regions of the ura3Δ3' and ura3Δ5' fragments with excision of the intervening ARG4 gene [ 16 ] . If CaCET1 were nonessential, then recombination of UAU1 into the second copy of CaCET1 (to generate cacet1::UAU1/cacet1::UAU1 ) followed by excisional recombination of ARG4 in one allele to restore URA3 (generating cacet1::UAU1/cacet1::URA3 ) would result in the selected Arg +Ura +phenotype with complete loss of the wild-type CaCET1 locus.
A gene flanked by required loci is less likely to be lost in an early large deletion than a gene flanked by nonessential loci.
Studies in yeast and Drosophila indicated that profilin is generally nonessential for cellular viability, although profilin mutants have phenotypes.
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