Words similar to nonessential
Example sentences for: nonessential
How can you use “nonessential” in a sentence? Here are some example sentences to help you improve your vocabulary:
Large deletions spanning multiple genes are possible only early in the reductive process, when many genes are nonessential.
Studies in yeast and Drosophila indicated that profilin is generally nonessential for cellular viability, although profilin mutants have phenotypes.
This frequency of homozygosity at a nonessential locus is similar to that reported by Mitchell's group (2 out of 30) for homozygous disruption of the C. albicans CDC25 gene [ 16 ] . These results confirm that the single-transformation test can, in our hands, be used to identify a nonessential gene and they underscore the inference from the data presented here that RNA triphosphatase is essential for growth of C. albicans.
Third, we grew 54 independent liquid cultures of the heterozygotes in nonselective medium and then selected for cells that were Arg +and Ura +. Uracil prototrophy requires restitution of the integrity of the disrupted ura3 gene of the UAU1 cassette by recombination between the overlapping regions of the ura3Δ3' and ura3Δ5' fragments with excision of the intervening ARG4 gene [ 16 ] . If CaCET1 were nonessential, then recombination of UAU1 into the second copy of CaCET1 (to generate cacet1::UAU1/cacet1::UAU1 ) followed by excisional recombination of ARG4 in one allele to restore URA3 (generating cacet1::UAU1/cacet1::URA3 ) would result in the selected Arg +Ura +phenotype with complete loss of the wild-type CaCET1 locus.
A gene flanked by required loci is less likely to be lost in an early large deletion than a gene flanked by nonessential loci.
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