Words similar to non-permissive
Example sentences for: non-permissive
How can you use “non-permissive” in a sentence? Here are some example sentences to help you improve your vocabulary:
npa1-1 and npa2-1 cells ceased growing after 3-4 hours at the non-permissive temperature.
In addition, glial scarring has long been suspected to contribute to the non-permissive environment encountered by regenerating axons in the CNS.
Both regions contained multiple open reading frames; however, we focused immediately on a single gene for each mutant, SEC13 for npa2-1 and SEC23 for npa1-1 . SEC13 was first identified in a screen for secretory mutants and encodes a protein of the COPII coat, which is required for vesicle budding from the ER [ 37 38 39 ] . More recently, it has been shown that a fraction of the total cellular Sec13p co-fractionates with NPCs in a distinct subcomplex with Nup84p, Nup85p, Nup120p, Nup145Cp and Seh1p [ 40 41 ] . SEC23 was also identified in a screen for secretory mutants, and it, too, is a component of the COPII coat [ 37 38 39 ] . Co-purification of Sec23p with intact NPCs has not been reported [ 5 ] ; however, Sec23p was recently isolated in Nup42p and Nup116p affinity chromatography strategies [ 42 ] . Expression constructs containing only SEC13 or SEC23 were transformed into the mutant strains and assayed for their ability to rescue growth at the non-permissive temperature.
Unless otherwise indicated, all studies were conducted at cell confluency and 14 days after inactivation of ts -SV-40 T at the non-permissive temperature (37°C) when wild-type podocytes are fully differentiated [ 27 28 ] .
However, when the genes encoding GFP-tagged Nups were crossed into these mutant backgrounds, all displayed GFP-Nup mislocalization under non-permissive conditions (Figure 7and data not shown).
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